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Free download in PDF Electrophoresis Objective Type Questions and Answers for competitive exams. These short objective type questions with answers are very important for competitive exams like IIT-JEE, AIIMS etc. as well as Board exams. These short solved questions or quizzes are provided by Gkseries.
(21)
Which of the following is used as a ‘tracking dye’ in SDS-PAGE of protein?
[A]
Bromophenol blue
[B]
Xylene cyanol
[C]
Orange G
[D]
All of these
(22)
For the better resolution of minute protein bands in SDS-PAGE, which of the following staining method is advised?
[A]
CBB Staining
[B]
Silver staining
[C]
Avidin staining
[D]
All of these
(23)
In a gel filtration column
[A]
smaller proteins enter the beads more readily
[B]
large proteins elute first
[C]
both (1) and (2)
[D]
large proteins enter the beads more readily
(24)
In isoelectric focusing, proteins are separated on the basis of their
[A]
relative content of positively charged residue only
[B]
relative content of negatively charged residue only
[C]
size
[D]
relative content of positively and negatively charged residue
Answer: relative content of positively and negatively charged residue
(25)
In SDS-PAGE, the protein sample is first
[A]
treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis
[B]
fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent
[C]
treated with a oxidizing agent and then with anionic detergent followed by fractionation by electrophoresis
[D]
none of the above
Answer: treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis
(26)
In an SDS-PAGE
[A]
proteins are denatured by the SDS
[B]
proteins have the same charge-to-mass ratio
[C]
smaller proteins migrate more rapidly through the gel
[D]
all of the above
(27)
In a native PAGE, proteins are separated on the basis of
[A]
net negative charge
[B]
net charge and size
[C]
net positive charges size
[D]
net positive charge
Answer: net charge and size
(28)
The role of urea in PAGE separation of DNA is to _______.
[A]
Act as anion
[B]
Act as cation
[C]
Helps to denature the DNA
[D]
Provide buffer stability of the gel
Answer: Helps to denature the DNA
(29)
In native-PAGE the separation of protein is influenced by ___________.
[A]
Charge of protein
[B]
Size of protein
[C]
pI of protein
[D]
Both (a) and (b)
(30)
In electrophoresis, the electrophoretic mobility (‘µ’) is determines the characteristics of migration of different biomolecules. Which of the following is not having any influence in ‘µ’?
[A]
Size of molecule
[B]
Shape of molecule
[C]
Molecular weight
[D]
Stereochemistry of molecule
Answer: Stereochemistry of molecule
31
Function of β-mercaptoethanol in SDS-PAGE is__________.
[A]
To give negative charges to amino acids in the proteins
[B]
For the oxidation of disulfide bonds in the proteins
[C]
For breaking hydrogen bonds in the proteins
[D]
For the reduction of disulfide bonds in the proteins
Answer: For the reduction of disulfide bonds in the proteins
32
The ratio of velocity (‘v’) of biomolecule in a medium under constant electric field (‘E’) is called ‘Electrophoretic mobility’ denoted as ‘µ’. ‘µ’ is mathematically expressed as:
[A]
µ = E/v
[B]
µ = 1/(Ev)
[C]
µ = VE
[D]
µ = v/E
33
Electrophoresis is not used for the separation of ________.
[A]
Nucleic acids
[B]
Proteins
[C]
Amino acids
[D]
Lipids
34
In SDS-PAGE of protein separation, one SDS molecule will binds to __________.
[A]
Every amino acid
[B]
Every two amino acids
[C]
Every three amino acids
[D]
Every Four amino acids
Answer: Every two amino acids
35
Glycerol is added to protein samples before they are loaded to the ‘wells’ of PAGE. The function of glycerol is to _______.
[A]
Stabilize protein structure
[B]
Provide density to the sample
[C]
Helps to bind SDS to the protein
[D]
Helps to reduce disulfide bonds by β-mercaptoethanol
Answer: Provide density to the sample
36
What does the electrophoresis apparatus consist of?
[A]
Gel, buffer chamber and fire pack
[B]
Buffer chamber and electrophoresis unit
[C]
Power pack and electrophoresis unit
[D]
Electrophoresis unit and gel separator
Answer: Power pack and electrophoresis unit
37
What cannot be a reason for using electrophoresis?
[A]
Comparing two sets of DNA
[B]
Organizing DNA by shape of backbone
[C]
Organizing DNA fragments from largest to smallest
[D]
Organizing DNA in order we can see
Answer: Organizing DNA by shape of backbone
38
If DNA is digested by endonucleases in four sites giving rise to fragments of which two are equal in length how many bands would be seen after electrophoresis?
[A]
3
[B]
4
[C]
5
[D]
Any of the above
39
Which of the following will migrate faster? The condition is the molecular weight of the following is equal.
[A]
Nicked circular DNA
[B]
Double stranded DNA
[C]
Single stranded DNA
[D]
Supercoiled circular DNA
Answer: Supercoiled circular DNA
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