{"id":159473,"date":"2023-09-15T12:58:59","date_gmt":"2023-09-15T07:28:59","guid":{"rendered":"https:\/\/www.gkseries.com\/blog\/?p=159473"},"modified":"2023-09-15T12:59:01","modified_gmt":"2023-09-15T07:29:01","slug":"the-product-of-complete-digestion-of-the-plasmid-shown-below-with-ecori-and-haeiii-was-purified","status":"publish","type":"post","link":"https:\/\/www.gkseries.com\/blog\/the-product-of-complete-digestion-of-the-plasmid-shown-below-with-ecori-and-haeiii-was-purified\/","title":{"rendered":"The product of complete digestion of the plasmid shown below with EcoRI and HaeIII was purified"},"content":{"rendered":"\n<p>Q. The product of complete digestion of the plasmid shown below with <em>Eco<\/em>RI and <em>Hae<\/em>III was purified and used as a template in a reaction containing Klenow fragment of DNA polymerase, dNTPs and [a-<sup>32<\/sup>P]-dATP in a suitable reaction buffer. The product thus obtained was purified and subjected to gel electrophoresis followed by autoradiography.<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full\"><img decoding=\"async\" width=\"393\" height=\"171\" src=\"https:\/\/www.gkseries.com\/blog\/wp-content\/uploads\/2023\/09\/Screenshot-906.png\" alt=\"\" class=\"wp-image-159474\" srcset=\"https:\/\/www.gkseries.com\/blog\/wp-content\/uploads\/2023\/09\/Screenshot-906.png 393w, https:\/\/www.gkseries.com\/blog\/wp-content\/uploads\/2023\/09\/Screenshot-906-300x131.png 300w\" sizes=\"(max-width: 393px) 100vw, 393px\" \/><\/figure>\n\n\n\n<p>The number of bands that will appear on the X-ray film is \u00a0\u00a0<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Q. The product of complete digestion of the plasmid shown below with EcoRI and HaeIII was purified and used as a template in a reaction containing Klenow fragment of DNA polymerase, dNTPs and [a-32P]-dATP in a suitable reaction buffer. The product thus obtained was purified and subjected to gel electrophoresis followed by autoradiography. The number [&hellip;]<\/p>\n","protected":false},"author":419,"featured_media":159475,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[5141],"tags":[5140],"class_list":["post-159473","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-gate","tag-gate-questions"],"_links":{"self":[{"href":"https:\/\/www.gkseries.com\/blog\/wp-json\/wp\/v2\/posts\/159473","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.gkseries.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.gkseries.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.gkseries.com\/blog\/wp-json\/wp\/v2\/users\/419"}],"replies":[{"embeddable":true,"href":"https:\/\/www.gkseries.com\/blog\/wp-json\/wp\/v2\/comments?post=159473"}],"version-history":[{"count":1,"href":"https:\/\/www.gkseries.com\/blog\/wp-json\/wp\/v2\/posts\/159473\/revisions"}],"predecessor-version":[{"id":159476,"href":"https:\/\/www.gkseries.com\/blog\/wp-json\/wp\/v2\/posts\/159473\/revisions\/159476"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.gkseries.com\/blog\/wp-json\/wp\/v2\/media\/159475"}],"wp:attachment":[{"href":"https:\/\/www.gkseries.com\/blog\/wp-json\/wp\/v2\/media?parent=159473"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.gkseries.com\/blog\/wp-json\/wp\/v2\/categories?post=159473"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.gkseries.com\/blog\/wp-json\/wp\/v2\/tags?post=159473"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}