Applications of Biotechnology MCQs | Applications of Biotechnology Multiple Choice Questions and Answers

(1) Which of the following is a correct statement
[A] ”Bt” in “Bt-cotton” indicates that it is a genetically modified organisms produced through biotechnology
[B] Somatic hybridization involves fusion of two complete plant cells carrying desired genes
[C] The anticoagulant hirudin is being produced from transgenic Brassica napus seed
[D] ”Flavr Savr”variety of tomato has enhanced the production of ethylene which improves its tastes its taste
Answer: The anticoagulant hirudin is being produced from transgenic Brassica napus seed
(2) Which bacteria is used as biopesticide first on the commercial scale in the world?
[A] Bacillus thuringiensis
[B] E.coli
[C] Pseudomonas aeruginosa
[D] Agrobacterium tumefaciens
Answer: Bacillus thuringiensis
(3) Gene recombinant technology is used for
[A] Vector less gene transfer into target cell
[B] Vector based gene transfer into target cell
[C] Direct transfer of DNA protein complex
[D] Liposome base direct gene transfer into target cell
Answer: Vector based gene transfer into target cell
(4) Transfer of DNA bands from an agrose gel to a nitrocellulose or nylon membrane is referred to as
[A] Western transfer
[B] Northern transfer
[C] Eastern transfer
[D] Southern transfer
Answer: Southern transfer
(5) Golden rice is a promising transgenic crop. When released for cultivation, it will help in
[A] Producing a petrol-like fuel from rice
[B] Alleviation of vitamin A
[C] Pest resistance
[D] Herbicide tolerance
Answer: Alleviation of vitamin A
(6) A genetically engineered micro-organism used successfully in bioremediation of oil spills is a species of
[A] Trichoderma
[B] Xanthomonass
[C] Bacillus
[D] Pseudomonas
Answer: Pseudomonas
(7) Blindness is prevented by use of which crop in poor countries?
[A] Golden rice
[B] Wheat
[C] Gram
[D] Pea
Answer: Golden rice
(8) Human insulin is being commercially produced from a transgenic species of
[A] Rhizobium
[B] Saccharomyces
[C] Escherichia
[D] None of these
Answer: Escherichia
(9) A transgenic food crop which may help in solving the problem of night blindness in developing countries is
[A] Bt soybean
[B] Golden rice
[C] Flavrsavrtomatoes
[D] Starlink maize
Answer: Golden rice
(10) Main objective of production/use of herbicide resistant GM crops is to
[A] Encourage eco-friendly herbicides
[B] Reduce herbicide accumulation in food articles for healt h safety
[C] Eliminate weeds from the field without the use of manual labour
[D] Eliminate weeds from the field without the use of herbicide
Answer: Reduce herbicide accumulation in food articles for healt h safety
(11) Genetically engineered bacteria are being employed for production of
[A] Thyroxine
[B] Human insulin
[C] Cortisol
[D] Epinephrine
Answer: Thyroxine
(12) Isolation of Bt gene from bacterium (Bacillus thuringiensis) was taken up in the year
[A] 1977
[B] 1980
[C] 1997
[D] 1990
Answer: 1980
(13) Which one of the following is commonly used in transfer of foreign DNA into crop plants?
[A] Meloidogyne incognita
[B] Agrobacterium tumefaciens
[C] Pencillium expansum
[D] Trichoderma harzianum
Answer: Agrobacterium tumefaciens
(14) What is true about Bt toxin?
[A] Bt toxin exists as active toxin in the Bacillius
[B] The activated toxin enters the ovaries of the pest to sterlise it and thus prevents its multiplication
[C] The concerned Bacillus has anti toxin
[D] The inactive prototoxin gets converted into active form in the insect gut
Answer: The inactive prototoxin gets converted into active form in the insect gut
(15) Transgenic plants are the ones
[A] Generated by introducing foreign DNA into a cell and regenerating a plant from the cell
[B] Produced after protoplast fusion in artificial medium
[C] Grown in artificial medium after hybridization in the field
[D] Produced by a somatic embryo in artificial medium
Answer: Generated by introducing foreign DNA into a cell and regenerating a plant from the cell
(16) The bacteria Bacillus thuringiensis is widely used in contemporary biology as
[A] Insecticide
[B] Agent for production of diary products
[C] Source of industrial enzyme
[D] Indicator of water pollution
Answer: Insecticide
(17) Golden rice is
[A] A variety of rice grown along the yellow river in china
[B] Long stored rice having yellow colour tint
[C] A transgenic rice having gene for β-carotene
[D] Wild variety of rice with yellow coloured grains
Answer: A variety of rice grown along the yellow river in china
(18) In RNAi,genes are silenced using
[A] ss DNA
[B] ds DNA
[C] ds RNA
[D] ss RNA
Answer: ds RNA
(19) The first clinical gene therapy was done for the treatment of
[A] AIDS
[B] Cancer
[C] Cystic fibrosis
[D] SCID
Answer: Cystic fibrosis
(20) ADA is an enzyme which is deficient in a genetic disorder SCID. What is the full form of ADA?
[A] Adenosine deoxyaminase
[B] Adenosine deaminase
[C] Aspartate deaminase
[D] Arginine deaminase
Answer: Adenosine deaminase

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