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Free download in PDF Applications of Biotechnology Objective Type Questions and Answers for competitive exams. These short objective type questions with answers are very important for Board exams as well as competitive exams like NEET, AIIMS, JIPMER etc. These short solved questions or quizzes are provided by Gkseries./p>
(1)
Silencing of a gene could be achieved through the use of
[A]
Short interfering RNA(RNAi)
[B]
Antisense RNA
[C]
By both
[D]
None of the above
(2)
Silencing of m-RNA has been used in producing transgenic plants resistant to
[A]
Bollworms
[B]
Nematodes
[C]
White rusts
[D]
Bacterial blights
(3)
Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produces(in the host cell)
[A]
Both sense and anti-sense RNA
[B]
A particular hormone
[C]
An antifeedant
[D]
A toxin protein
Answer: Both sense and anti-sense RNA
(4)
Amplification of gene of interest by using DNA polymerase may go upto
[A]
0.1 million times
[B]
1.0 million times
[C]
1.0 billion times
[D]
1.0 trillion times
Answer: 1.0 billion times
(5)
Which of the following Bt crops is being grown in India by the farmers?
[A]
Cotton
[B]
Brinjal
[C]
Soybean
[D]
Maize
(6)
The first human hormone produced by recombinant DNA technology is
[A]
Insulin
[B]
Estrogen
[C]
Thyroxin
[D]
Progesterone
(7)
An analysis of chromosomal DNA using the hybridization technique does not use
[A]
Electrophoresis
[B]
Blotting
[C]
Autoradiography
[D]
PCR
(8)
Bt cotton is not:
[A]
A GM plant
[B]
Insect resistant
[C]
A bacterial gene expressing system
[D]
Resistant to all pesticides
Answer: Resistant to all pesticides
(9)
C-peptide of human insulin is:
[A]
A part of mature insulin molecule
[B]
Responsible for formation of disulphide bridges
[C]
Removed during maturation of pro-insulin to insulind
[D]
Responsible for its biological activity
Answer: Removed during maturation of pro-insulin to insulind
(10)
GEAC stands for:
[A]
Genome Engineering Action Committee
[B]
Ground Environment Action Committee
[C]
Genetic Engineering Approval Committee
[D]
Genetic and Environment Approval committee
Answer: Genetic Engineering Approval Committee
(11)
α -1 antitrypsin is:
[A]
An antacid
[B]
An enzyme
[C]
Used to treat arthritis
[D]
Used to treat emphysema
Answer: Used to treat emphysema
(12)
The site of production of ADA in the body is:
[A]
Bone marrow
[B]
Lymphocytes
[C]
Blood plasma
[D]
Monocytes
(13)
A protoxin is:
[A]
A primitive toxin
[B]
A denatured toxin
[C]
Toxin produced by protozoa
[D]
Inactive toxin
(14)
The trigger for activation of toxin of Bacillus thuringiensis is:
[A]
Acidic pH of stomach
[B]
High temperature
[C]
Alkaline pH of gut
[D]
Mechanical action in the insect gut
Answer: Alkaline pH of gut
(15)
In RNAi, genes are silenced using:
[A]
ss DNA
[B]
ds DNA
[C]
ds RNA
[D]
ss RNA
(16)
‘Molecular scissor’ used in genetic engineering is
[A]
Restriction endonuclease
[B]
DNA polymerase
[C]
DNA ligase
[D]
Helicase
Answer: Restriction endonuclease
(17)
Plants are genetically engineered with novel genes by
[A]
Recombination breeding
[B]
Protoplast fusion
[C]
Recombinant DNA technology
[D]
Embryo rescue technique
Answer: Recombinant DNA technology
(18)
Maximum application of animal culture technology today is in the production
[A]
Insulin
[B]
Interferons
[C]
Edible proteins
[D]
Vaccines
(19)
Restriction enzymes were discovered by
[A]
Smith and Nathans
[B]
Alexander Fleming
[C]
Berg
[D]
None
Answer: Smith and Nathans
(20)
Bacteria protect themselves from viruses by fragmenting viral DNA with
[A]
Ligase
[B]
Endonuclease
[C]
Exonuclease
[D]
Gyrase
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