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Free download in PDF Applications of Biotechnology Short Questions and Answers for competitive exams. These short objective type questions with answers are very important for Board exams as well as competitive exams like NEET, AIIMS, JIPMER etc. These short solved questions or quizzes are provided by Gkseries./p>
(1)
Klenow fragment is derived from
[A]
DNA Ligase
[B]
DNA Pol-I
[C]
DNA Pol-II
[D]
Reverse Transcriptase
(2)
Southern blotting is
[A]
Attachment of probes to DNA fragments
[B]
Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet
[C]
Comparison of DNA fragments to two sources
[D]
Transfer of DNA fragments to electrophoretic gel from cellulose membrane
Answer: Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet
(3)
ELISA is
[A]
Using radiolabelled second antibody
[B]
Usage of RBCs
[C]
Using complement-mediated cell lysis
[D]
Addition of substrate that is converted into a coloured end product
Answer: Addition of substrate that is converted into a coloured end product
(4)
The Golden Rice variety is rich in
[A]
Vitamin C
[B]
Β-carotene and ferritin
[C]
Biotin
[D]
Lysine
Answer: Β-carotene and ferritin
(5)
The DNA fragments have sticky ends due to
[A]
Endonuclease
[B]
Unpaired bases
[C]
Calcium ions
[D]
Free methylation
(6)
Plasmids are used as cloning vectors for which of the following reasons?
[A]
Can be multiplied in culture
[B]
Self-replication in bacterial cells
[C]
Can be multiplied in laboratories with the help of enzymes
[D]
Replicate freely outside bacterial cells
Answer: Self-replication in bacterial cells
(7)
The human genome project was launched in the year
[A]
1980
[B]
1973
[C]
1990
[D]
1989
(8)
The vaccines prepared through recombinant DNA technology are
[A]
Third generation vaccines
[B]
First-generation vaccines
[C]
Second-generation vaccines
[D]
None
Answer: Third generation vaccines
(9)
Which is a genetically modified crop?
[A]
Bt-cotton
[B]
Bt-brinjal
[C]
Golden rice
[D]
All
(10)
PCR technique was invented by
[A]
Karry Mullis
[B]
Boyer
[C]
Sanger
[D]
Cohn
(11)
The first transgenic plant to be produced is
[A]
Brinjal
[B]
Tobacco
[C]
Rice
[D]
Cotton
(12)
RNA interference helps in
[A]
Cell proliferation
[B]
Micropropagation
[C]
Cell defence
[D]
Cell differentiation
(13)
Which of the following is the quality of improved transgenic basmati rice?
[A]
Gives high yield but no characteristic aroma
[B]
Gives high yield and is rich in vitamin A
[C]
Does not require chemical fertilizers and growth hormones
[D]
Resistant to insects and diseases
Answer: Gives high yield and is rich in vitamin A
(14)
The first clinical application of gene therapy over a 4 year old girl was for
[A]
Adenosine deaminase deficiency
[B]
Adenosine deficiency
[C]
Growth deficiency
[D]
Adenine deficiency
Answer: Adenosine deaminase deficiency
(15)
Excision and insertion of a gene is called
[A]
Biotechnology
[B]
Genetic engineering
[C]
Cytogenetics
[D]
Gene therapy
Answer: Genetic engineering
(16)
The expression of a transgene in the target tissue is identified by a
[A]
Transgene
[B]
Promoter
[C]
Enhancer
[D]
Reporter
(17)
———– is used as a vector for cloning into higher organisms
[A]
Retrovirus
[B]
Baculovirus
[C]
Salmonella typhimurium
[D]
Rhizopus nigricans
(18)
Which bacterium is used in the production of insulin by genetic engineering?
[A]
Saccharomyces
[B]
Rhizobium
[C]
Escherichia
[D]
Mycobacterium
(19)
Golden rice is a transgenic crop of the future with the following improved trait.
[A]
Insect resistance
[B]
High protein content
[C]
High vitamin A content
[D]
High lysine content
Answer: High vitamin A content
(20)
DNA fingerprinting refer to
[A]
Techniques used for identification of fingerprints of individuals
[B]
Molecular analysis of profiles of DNA samples
[C]
Analysis of DNA samples using imprinting devices
[D]
Techniques used for molecular analysis of different specimens of DNA
Answer: Molecular analysis of profiles of DNA samples
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