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Free download in PDF Molecular Biology Techniques Multiple Choice Questions and Answers for competitive exams. These short objective type questions with answers are very important for Board exams as well as competitive exams. These short solved questions or quizzes are provided by Gkseries.
(1)
Southern Hybridization is
[A]
Used To Identify A Specific DNA
[B]
Used To Identify Both DNA And RNA
[C]
Used To Identify A Specific Protein
[D]
Used To Identify A Specific RNA
Answer: Used To Identify A Specific DNA
(2)
Applications Of Southern Blotting Includes
[A]
Identification Of Transferred Genes
[B]
DNA Fingerprinting
[C]
Preparation Of RFLP Maps
[D]
All Of These
(3)
First DNA Glycosylase Enzyme Discovered Is ___________.
[A]
Thymine DNA Glycosylase
[B]
Adenine DNA Glycosylase
[C]
Methyl Adenine Glycosylase
[D]
Uracil DNA Glycosylase
Answer: Uracil DNA Glycosylase
(4)
The Enzyme Photolyase Repair The Thymine Dimer In The DNA By __________ Mechanism.
[A]
Free Radical Mechanism
[B]
Adduct Formation
[C]
Oxidation Mechanism
[D]
Direct Bond Breaking
Answer: Free Radical Mechanism
(5)
The Function Of Uvrc In NER Mechanism Is_________.
[A]
Catalyze The Incision At The 3’ End Of The Lesion
[B]
Catalyze The Incision At The 5’ Side Of The Lesion
[C]
To Identify The Lesion In The DNA
[D]
Helicase Activity To Unwind The DNA
Answer: Catalyze The Incision At The 5’ Side Of The Lesion
(6)
The Catalytic Activity Of Photolyase Enzyme In The Bacterial Cells Is Activated By _____.
[A]
Visible Light Near Blue Region
[B]
Visible Light Near Red Region
[C]
IR Light
[D]
UV Light
Answer: Visible Light Near Blue Region
(7)
Photolyase Enzyme Involved In The Photoreactivation Can Remove ______.
[A]
UV Induced Thymine Dimers
[B]
Single Strand Breaks In DNA
[C]
UV Induced Double Strand Breaks
[D]
Mismatches In DNA
Answer: UV Induced Thymine Dimers
(8)
Which Of The Following Gene Is NOT Involved In Mismatch Repair System In Bacteria?
[A]
Mutl
[B]
Muts
[C]
Muta
[D]
None of these
(9)
Which Enzyme Is Responsible For Photoreactivation Of DNA:
[A]
Photolyase
[B]
Photo-Oxidase
[C]
Photoligase
[D]
Photoreductase
(10)
Which Of The Following DNA Repair Mechanism Is Known As The ‘Cut And Patch Mechanism’?
[A]
Nucleotide Excision Repair
[B]
Mismatch Repair
[C]
Photoreactivation
[D]
None of these
Answer: Nucleotide Excision Repair
(11)
Which Of The Following Enzyme(S) Is Involved Base Excision Repair?
[A]
DNA Glycosylase
[B]
AP Endonuclease
[C]
AP Exonuclease
[D]
Both (A) And (B)
(12)
The Technique Used To Identify Specific DNA Sequence In Bacterial Colonies Is
[A]
Western Blotting
[B]
Dot Blot Technique
[C]
In Situ Hybridization
[D]
Colony Hybridization
Answer: Colony Hybridization
(13)
Which Of The Following Statements Are True Regarding Southern Blotting
[A]
DNA-DNA Hybridization Is The Basis
[B]
The Transfer Of DNA Fragments From Gel To Nitrocellulose Membrane Is Called Blotting
[C]
Developed By E.M.Southern
[D]
All Of These
(14)
The Technique Used To Detect The Presence Of DNA Or RNA In A Non-Fractionated DNA Sample Is
[A]
Dot Blot Technique
[B]
Western Blotting
[C]
Colony Hybridization
[D]
None of these
Answer: Dot Blot Technique
(15)
Northern Hybridization Is
[A]
Used To Identify Both DNA And RNA
[B]
Used To Identify A Specific RNA
[C]
Used To Identify A Specific Protein
[D]
Used To Identify A Specific DNA
Answer: Used To Identify A Specific RNA
(16)
Southern Hybridization is
[A]
Used To Identify Both DNA And RNA
[B]
Used To Identify A Specific DNA
[C]
Used To Identify A Specific RNA
[D]
Used To Identify A Specific Protein
Answer: Used To Identify A Specific DNA
(17)
For Glycoproteins, Most Commonly Used Probe Is
[A]
Interferons
[B]
Antigens
[C]
Lectin
[D]
Antibody
(18)
ELISA Is Based On
[A]
Lectin- Antibody Interaction
[B]
Antigen-Protein Intercation
[C]
Antigen-Antibody Interaction
[D]
All Of These
Answer: Antigen-Antibody Interaction
(19)
The Technique Used To Locate Specific Genes In Chromosomes Is
[A]
Colony Hybridization
[B]
Dot Blot Technique
[C]
Western Blotting
[D]
In Situ Hybridization
Answer: In Situ Hybridization
(20)
The Separation Technique Of Charged Molecules Under The Influence Of Electric Current Is Called
[A]
Western Blotting
[B]
Colony Hybridization
[C]
Dot Blot Technique
[D]
Electrophoresis
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