Download PDF
Free download in PDF Principles and Processes of Biotechnology Multiple Choice Questions and Answers for competitive exams. These short objective type questions with answers are very important for Board exams as well as competitive exams like NEET, AIIMS, JIPMER etc. These short solved questions or quizzes are provided by Gkseries./p>
(1)
A singe strand of nucleic acid tagged with a radioactive molecules is called
[A]
Vector
[B]
Selectable marker
[C]
Plasmid
[D]
Probe
(2)
Which one of the following is a case of wrong matching ?
[A]
Somatic Hybridization- Fusion of two diverse cells
[B]
Vector DNA- Site for tRNA synthesis
[C]
Micropropagation- in vitro production of plants in large numbers
[D]
Callus- Unorganised mass of cells produced in tissue culture
Answer: Vector DNA- Site for tRNA synthesis
(3)
Which one is a true statement regarding DNA polymerase used in PCR ?
[A]
It is used to ligate introduced DNA in recipients cells
[B]
It serves as a selectable marker
[C]
It is isolated from a virus
[D]
It remains active at high temperature
Answer: It remains active at high temperature
(4)
For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of
[A]
Silver or platinum
[B]
Platinum or zinc
[C]
Silicon or platinum
[D]
Gold or trungsten
Answer: Gold or trungsten
(5)
Biolistics (gene-gun) is suitable
[A]
Disarming pathogen vector
[B]
Transformation of plant cells
[C]
Constructing recombinant DNA by joining with vectors
[D]
DNA fingerprinting
Answer: Transformation of plant cells
(6)
In genetic engineering, the antibiotics are used
[A]
As selectable markers
[B]
To select healthy vectors
[C]
As sequence from where replication starts
[D]
To keep the culture free of infection
Answer: As selectable markers
(7)
Which one of the following represents a palindromic sequence in DNA ?
[A]
5’-GAATTC-3 3’-CTTAAG-5’
[B]
5’-CCAATG-3’
[C]
3’-GAATCC-5’
[D]
5’-CATTAG-3’ 3’-GATAAC-5’
Answer: 5’-GAATTC-3 3’-CTTAAG-5’
(8)
The colonies of recombinant bacteria appear white in contrast to blue colonies of non- recombinant bacteria because of
[A]
Insertional inactivation of alpha galactosidese in recombinant bacteria
[B]
Inactivation of glycosides enzyme in recombinant bacteria
[C]
Non-recombinant bacteria containing beta galactosidase
[D]
Insertional inactivation of alpha galactosidase
Answer: Non-recombinant bacteria containing beta galactosidase
(9)
Which of the following is not correctly matched for the organism and its cell wall degrading enzyme ?
[A]
Algae - Methylase
[B]
Fungi - Chitinase
[C]
Bacteria - Lysozyme
[D]
Plant cells – Cellulase
Answer: Algae - Methylase
(10)
DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by
[A]
Electrophoresis
[B]
Restriction mapping
[C]
Centrifugation
[D]
Polymerase chain reaction
(11)
An analysis of chromosomal DNA using the southern hybridization technique does not use
[A]
Electrophoresis
[B]
Blotting
[C]
Autoradiography
[D]
PCR
(12)
In vitro clonal propagation in plants is characterized by
[A]
PCR and RAPD
[B]
Northern blotting
[C]
Electrophoresis and HPLC
[D]
Microscopy
(13)
Which vector can be clone only a small fragment of DNA ?
[A]
Bacterial artificial chromosome
[B]
Yeast artificial chromosome
[C]
Plasmid
[D]
Cosmid
(14)
Commonly used vectors for human genome sequencing are
[A]
T- DNA
[B]
BAC and YAC
[C]
Expression vectors
[D]
T/A cloning vectors
(15)
Which of the following is a plasmid ?
[A]
pBR322
[B]
BamH-I
[C]
Hind-III
[D]
EcoRI
(16)
Restriction endonucleases are must widely used in recombinant DNA technology. They are obtained from
[A]
Bacteriophages
[B]
Bacterial cells
[C]
Plasmids
[D]
All Prokaryotie Cells
(17)
Viral genome incorporated into host DNA is called
[A]
Prophase
[B]
Prophage
[C]
Bacteriophage
[D]
None of these
(18)
Two microbes found to be very useful in genetic engineering are
[A]
Crown gall bacterium and Conorhabditis elegens
[B]
Escherichia coli to Agrobacterium tumifaciens
[C]
Vibria choleraeand a tailed bacteriphage
[D]
Dipococens species and psendomonasap
Answer: Escherichia coli to Agrobacterium tumifaciens
(19)
Who disconnected recombinant DNA technoledge ?
[A]
Har Gobind Khorana
[B]
James Watson & Francis Crick
[C]
Stanly Cohen & Herbert Boyer
[D]
Watter Sutton
Answer: Stanly Cohen & Herbert Boyer
(20)
Find out the wrong statement ?
[A]
Mobile genetic element, Transposons were visualized by Barbara Mc Clintock
[B]
Udder cell a somatic cell is used to produce the cloned sheep by nuclear transplantation method
[C]
Dr. Ian Wilmut produced a cloned sheep called Dolley
[D]
DNA ligases are used to cleave a DNA molecule
Answer: DNA ligases are used to cleave a DNA molecule
Take Mock Tests