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Free download in PDF Principles and Processes of Biotechnology Objective Type Questions and Answers for competitive exams. These short objective type questions with answers are very important for Board exams as well as competitive exams like NEET, AIIMS, JIPMER etc. These short solved questions or quizzes are provided by Gkseries./p>
(1)
The DNA fragments have sticky ends due to
[A]
Endonuclease
[B]
Unpaired bases
[C]
Calcium ions
[D]
Free methylation
(2)
Plasmids are used as cloning vectors for which of the following reasons?
[A]
Can be multiplied in culture
[B]
Self-replication in bacterial cells
[C]
Can be multiplied in laboratories with the help of enzymes
[D]
Replicate freely outside bacterial cells
Answer: Can be multiplied in laboratories with the help of enzymes
(3)
The human genome project was launched in the year
[A]
1980
[B]
1973
[C]
1990
[D]
1989
(4)
The vaccines prepared through recombinant DNA technology are
[A]
Third generation vaccines
[B]
First-generation vaccines
[C]
Second-generation vaccines
[D]
None
Answer: Third generation vaccines
(5)
Which is a genetically modified crop?
[A]
Bt-cotton
[B]
Bt-brinjal
[C]
Golden rice
[D]
All
(6)
PCR technique was invented by
[A]
Karry Mullis
[B]
Boyer
[C]
Sanger
[D]
Cohn
(7)
The first transgenic plant to be produced is
[A]
Brinjal
[B]
Tobacco
[C]
Rice
[D]
Cotton
(8)
RNA interference helps in
[A]
Cell proliferation
[B]
Micropropagation
[C]
Cell defence
[D]
Cell differentiation
(9)
Which of the following is the quality of improved transgenic basmati rice?
[A]
Gives high yield but no characteristic aroma
[B]
Gives high yield and is rich in vitamin A
[C]
Does not require chemical fertilizers and growth hormones
[D]
Resistant to insects and diseases
Answer: Gives high yield and is rich in vitamin A
(10)
The first clinical application of gene therapy over a 4 year old girl was for
[A]
Adenosine deaminase deficiency
[B]
Adenosine deficiency
[C]
Growth deficiency
[D]
Adenine deficiency
Answer: Adenosine deaminase deficiency
(11)
Excision and insertion of a gene is called
[A]
Biotechnology
[B]
Genetic engineering
[C]
Cytogenetics
[D]
Gene therapy
Answer: Genetic engineering
(12)
The expression of a transgene in the target tissue is identified by a
[A]
Transgene
[B]
Promoter
[C]
Enhancer
[D]
Reporter
(13)
———– is used as a vector for cloning into higher organisms
[A]
Retrovirus
[B]
Baculovirus
[C]
Salmonella typhimurium
[D]
Rhizopus nigricans
(14)
Which bacterium is used in the production of insulin by genetic engineering?
[A]
Saccharomyces
[B]
Rhizobium
[C]
Escherichia
[D]
Mycobacterium
(15)
Restriction endonuclease
[A]
Synthesizes DNA
[B]
Cuts the DNA molecule randomly
[C]
Cuts the DNA molecule at specific sites
[D]
Restricts the synthesis of DNA inside the molecules
Answer: Cuts the DNA molecule at specific sites
(16)
Gel electrophoresis is used for
[A]
Construction of recombinant DNA by joining with cloning vectors
[B]
Isolation of DNA molecules
[C]
Cutting of DNA into fragments
[D]
Separation of DNA fragments according to their size
Answer: Separation of DNA fragments according to their size
(17)
The linking of antibiotic resistance gene with the plasmid vector become possible with
[A]
DNA polymerase
[B]
Exonucleases
[C]
DNA ligase
[D]
Endonucleases
(18)
Polyethylene glycol method is used for
[A]
Biodiesel production
[B]
Seedless fruit production
[C]
Energy production from swage
[D]
Gene transfer without a vector
Answer: Gene transfer without a vector
(19)
Which one of the following is used as vector for cloning genes into higher organisms ?
[A]
Baculovirus
[B]
Salmonella typhimurium
[C]
Rhizopus nigricans
[D]
Retrovirus
(20)
DNA or RNA segment tagged with a radioactive molecules is called
[A]
Vector
[B]
Probe
[C]
Clone
[D]
Plasmid
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