Protein Synthesis Objective Type Questions and Answers

(21) The preinitiation complex in prokaryotes include

[A] Intiation factors, mRNA, 30 S subunit, GTP

[B] Intiation factors, mRNA, 30 S subunit, 50 S subunit, ATP

[C] Intiation factors, mRNA, 50 S subunit, GTP

[D] Intiation factors, 30 S subunit, 50 S subunit, ATP

Answer: Intiation factors, mRNA, 30 S subunit, GTP

(22) Translocation in protein synthesis begins with the

[A] movement of dipeptidyl tRNA from A-site to P-site

[B] movement of tRNA from A-site to P-site

[C] movement of tRNA from P-site to A-site

[D] movement of tRNA from P-site to E-site

Answer: movement of dipeptidyl tRNA from A-site to P-site

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(23) The amino acids are assembled into polypeptide chains on

[A] Ribosome

[B] DNA Template

[C] Mrna

[D] Nucleus

Answer: Ribosome

(24) Which of the following release factor recognizes stop codons UGA and UAA?

[A] RF2

[B] RF3

[C] Sigma factor

[D] RF1

Answer: RF2

(25) Teminism is

[A] Reverse transcription

[B] DNA replication

[C] RNA replication

[D] None of these

Answer: Reverse transcription

(26) During trancription, RNA polymerase binds to DNA site

[A] Receptor

[B] Enhancer

[C] Regulator

[D] Promoter

Answer: Promoter

(27) Which one is correct matching?

[A] anticodon- site of tRNA having complementary bases to a codon of mRNA

[B] transcription- synthesis of protein

[C] translation- process of mRNA carrying information from nucleus to ribosome

[D] None of these

Answer: anticodon- site of tRNA having complementary bases to a codon of mRNA

(28) What is the size of the prokaryotic ribosome?

[A] 70S

[B] 60S

[C] 80S

[D] 40S

Answer: 70S

(29) Which of the following is not true to the nature of the genetic code?

[A] Codons are overlapping

[B] Codons are commaless

[C] Codons are universal

[D] Codon is triplet

Answer: Codons are overlapping

(30) Who explained the wobble hypothesis?

[A] Watson and Crick

[B] Nirenberg

[C] Darwin

[D] Samuel B. Weiss

Answer: Watson and Crick

31 Name the inhibitor which blocks translation in both prokaryotes as well as eukaryotes?

[A] Puromycin

[B] Streptomycin

[C] Chlorophenicol

[D] Tetracyclinee

Answer: Puromycin

32 Bacteria have

[A] 80S ribosomes

[B] 60S ribosomes

[C] 50S ribosomes

[D] None of above

Answer: 70S ribosomes

33 Which of the following catalyzes ADP-ribosylation of a diphthamide residue?

[A] Diphtheria toxin

[B] Cycloheximide

[C] Chloramphenicol

[D] Ricin

Answer: Diphtheria toxin

34 Which of the following inhibits initiation at higher concentrations?

[A] Streptomycin

[B] Diphtheria toxin

[C] Chloramphenicol

[D] Ricin

Answer: Streptomycin

35 The enzyme involved in activation of serine is

[A] seryl-tRNA synthetase

[B] peptidyl transfrase

[C] amino acyl-tRNA synthetase

[D] peptide polymerase

Answer: seryl-tRNA synthetase

36 Peptide bond formation between amino acids of growing polypeptide chain is catalysed by

[A] peptide polymerase

[B] Peptidyl synthetase

[C] amino acyl-tRNA synthetase

[D] peptidyl transfrase

Answer: peptidyl transfrase

37 Peptidyl transfrase involved in peptide bond formation is located in the

[A] larger subunit of ribosome

[B] smaller subunit of ribosome

[C] 3’ region of tRNA

[D] Near Shine Dalgarno sequence

Answer: larger subunit of ribosome

38 Which of the following elongation factor is called as translocase?

[A] EFG

[B] EF2

[C] both a and b

[D] EF-Tu and EF-Ts

Answer: both a and b

39 In eukaryotes transcription occurs

[A] Cytoplasm

[B] Cytosol

[C] Nucleus

[D] All the above

Answer: Nucleus

40 Antibiotic inhibiting translation in eukaryotes is

[A] Puromycin

[B] Tetracycline

[C] Pencillin

[D] Chloromycin

Answer: Puromycin

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