July 2023

Q. Which of the following statements are CORRECT about the function of fetal bovine serum in animal cell culture? P. It stimulates cell growth Q. It enhances cell attachment C. It provides hormones and minerals D. It maintains pH at 7.4 (A) P and Q only (B) P and S only (C) P, Q and […]

Q. The hexapeptide P has an isoelectric point (pI) of 6.9. Hexapeptide Q is a variant of P that contains valine instead of glutamate at position 3. The two peptides are analyzed by polyacrylamide gel electrophoresis at pH 8.0. Which one of the following statements is CORRECT? (A) P will migrate faster than Q towards

Q. Which one of the following is coded by the ABO blood group locus in the human genome? (A) Acyl transferase (B) Galactosyltransferase (C) Transposase (D) β-Galactosidase Ans: Galactosyltransferase Solution: (B) Galactosyltransferase The ABO blood group locus in the human genome codes for the enzyme Galactosyltransferase. This enzyme is responsible for the transfer of specific

Q. Which of the following statements are CORRECT when a protein sequence database is searched using the BLAST algorithm? P. A larger E-value indicates higher sequence similarity Q. E-value < 10-10 indicates sequence homology R. A higher BLAST score indicates higher sequence similarity S. E-value > 1010 indicates sequence homology (A) P, Q and R

Q. Which one of the following need NOT be conserved in a biochemical reaction? (A) Total mass (B) Total moles (C) Number of atoms of each element (D) Total energy Ans: Total moles

Q. Which one of the following is catabolized during endogenous metabolism in a batch bacterial cultivation? (A) internal reserves (B) extracellular substrates (C) extracellular products (D) toxic substrates Ans: internal reserves Solution: During endogenous metabolism in a batch bacterial cultivation, internal reserves are catabolized. Endogenous metabolism occurs when the extracellular substrates in the culture medium

Q. Which one of the following can NOT be a limiting substrate if Monod’s growth kinetics is applicable? (A) Extracellular carbon source (B) Extracellular nitrogen source (C) Dissolved oxygen (D) Intracellular carbon source Ans: Intracellular carbon source Solution: In Monod’s growth kinetics, the rate of microbial growth is described by the Monod equation, which considers

Q. Which one of the following statements is CORRECT for enzyme catalyzed reactions? (𝛥𝐺 is Gibbs free energy change, 𝐾𝑒𝑞 is equilibrium constant) (A) Enzymes affect 𝛥𝐺, but not 𝐾𝑒𝑞 (B) Enzymes affect 𝐾𝑒𝑞, but not 𝛥𝐺 (C) Enzymes affect both 𝛥𝐺 and 𝐾𝑒𝑞 (D) Enzymes do not affect 𝛥𝐺 or 𝐾𝑒𝑞 Ans: Enzymes affect