Culture Media MCQs

Q. Which of the following is a function of cryoprotective agents? to trap the liquid nitrogen for long-term preservation of cultures prevents formation of ice prevents cell damage due to ice crystal formation Answer: prevents cell damage due to ice crystal formation

Q. Nichrome loop wire is used in which of the following techniques? Streak-plate Pour-plate Spread-plate Roll-tube technique Answer: Streak-plate

Q. Which device is used to pick a single bacterial cell from a mixed culture? Micromanipulator Micropipette Microscope Microprobe Answer: Micromanipulator

Q. Which of the following is used as a solidifying agent for media? Agar Yeast extract Beef extract Peptone Answer: Agar

Q. The isolation of gonorrhea-causing organism, Neisseria gonorrhoeae by the use of certain antibiotics in media is an example of which of the following? Selective media Assay media Differential media Enriched media Answer: Selective media

Q. Which of the following instrument is used for sterilizing the media after it has been prepared? Incubator Inoculum Needle Laminar Air Flow Chamber Autoclave Answer: Autoclave

Q. Which of the following bacteria requires nicotinic acid as a growth factor in their media? Nitrosomonas sp coli Proteus vulgaris none of the above Answer: Proteus vulgaris

Q. Which of the following are functions of Maintenance Media? used for the maintenance of the viability and physiological characteristics used for determining the type of growth produced by bacteria used for assay of vitamins,amino acids used for determining the bacterial content Answer: used for the maintenance of the viability and physiological characteristics

Q. Which of the following is a Complex media for fungal growth? Potato Dextrose Agar(PDA) media Mac Conkey Agar media Nutrient broth Luria-Bertani media Answer: Potato Dextrose Agar(PDA) media

Q. Nutrient broth, a liquid media contains beef extract and peptone respectively in how much amounts? 0.3%, 0.5% 0.7%, 0.3% 0.2%, 0.4% 0.1%, 0.6% Answer: 0.3%, 0.5%