Q. Proteins are separated in an SDS-PAGE experiment on the basis of their positively charged side chains molecular weight negatively charged side chains different isoelectric points Answer: molecular weight
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Q. Electrophoresis of histones and myoglobin under non-denaturing conditions (pH = 7.0) results in both proteins migrate to the anode histones migrate to the anode and myoglobin migrates to the cathode histones migrate to the cathode and myoglobin migrates to the anode both proteins migrate to the cathode Answer: histones migrate to the cathode and
Q. Proteins can be visualized directly in gels by staining them with the dye using electron microscope only measuring their molecular weight none of these Answer: staining them with the dye
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Q. In SDS-PAGE, separation is based on molecular weight shape charge all of the above Answer: molecular weight
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Q. The electrophoresis technique that used isoelectric focusing is AGE PFGE 2D-PAGE SDS-PAGE Answer: 2D-PAGE
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Q. What is the role of SDS in SDS-PAGE? protein denaturing and imparting net negative charge imparting overall negative charge to the protein imparting equal mass to all proteins protein unfolding and imparting net positive charge Answer: protein denaturing and imparting net negative charge
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Q. Which is the technique suited for the separation of large DNA fragments? AGE PAGE PFGE SDS-PAGE Answer: PFGE
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Q. Electrophoresis cell or electrophoresis apparatus consists of power pack and electrophoresis unit Elctrophoresis unit and DNA separator buffer chamber and Elctrophoresis unit Gel, buffer chamber and power pack Answer: power pack and electrophoresis unit
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Q. The most common type of gel used for DNA separation is Agar Polyacrylamide Agarose All of the above Answer: Agarose
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Q. Which of the following statement is true regarding migration of bio molecules? the rate of migration is directly proportional to the current the rate of migration is inversely proportional to current the rate of migration is directly proportional to the resistance of the medium Low voltage is used for the separation of high molecular
Which of the following statement is true regarding migration of bio molecules? Read More »
