Q. Consider a simple uni-substrate enzyme that follows Michaelis-Menten kinetics. When the enzyme catalyzed reaction was carried out in the presence of 10 nM concentration of an inhibitor, there was no change in the maximal velocity. However, the slope of the Lineweaver-Burk plot increased 3-fold. The dissociation constant for the enzyme-inhibitor complex (in nM) is […]
Q. First order deactivation rate constants for soluble and immobilized amyloglucosidase enzyme are 0.03 min-1 and 0.005 min-1, respectively. The ratio of half-life of the immobilized enzyme to that of the soluble enzyme is (rounded off to the nearest integer) Ans: 6 to 6
Q. Consider the following alignment of two DNA sequences: Assuming an affine gap scoring scheme of an identity matrix for substitution, a gap initiation penalty of 1 and a gap extension penalty of 0.1, the score of the alignment is (up to one decimal place) Ans: 1.8 to 1.9
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Q. At the end of a batch culture, glucose solution is added at a flow rate of 200 ml/h. If the culture volume after 2 h of glucose addition is 1000 ml, the initial culture volume (in ml) is Ans: 600 to 600
At the end of a batch culture, glucose solution is added at a flow rate of 200 ml/h Read More »
Q. Yeast converts glucose to ethanol and carbon dioxide by glycolysis as per the following reaction: Assuming complete conversion, the amount of ethanol produced (in g) from 200 g of glucose is (up to two decimal places) Ans: 102.00 to 103.00
Q. In a chemostat, the feed flow rate and culture volume are 100 ml/h and 1.0 L, respectively. With glucose as substrate, the values of µmax and Ks are 0.2 h-1 and 1 g/L, respectively. For a glucose concentration of 10 g/L in the feed, the effluent substrate concentration (in g/L) is Ans: 1
Q. A microbial strain is cultured in a 100 L stirred fermenter for secondary metabolite production. If the specific rate of oxygen uptake is 0.4 h-1 and the oxygen solubility in the broth is 8 mg/L, then the volumetric mass transfer coefficient (KLa) (in s-1) of oxygen required to achieve a maximum cell concentration of
Q. An aqueous solution containing 6.8 mg/L of an antibiotic is extracted with amyl acetate. If the partition coefficient of the antibiotic is 170 and the ratio of water to solvent is 85, then the extraction factor is Ans: 2 to 2
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Q. 5′ capping of mRNA transcripts in eukaryotes involves the following events: P. Addition of GMP on the 5¢ end Q. Removal of g-phosphate of the triphosphate on first base at the 5¢ end C. 5′-5′ linkage between GMP and the first base at 5¢ end S. Addition of methyl group to N7 position of
5′ capping of mRNA transcripts in eukaryotes involves the following events: Read More »
Q. Match the industrial products mentioned in Group I with their producer organisms in Group II Group I Group II P. Citric acid 1. Trichoderma viride Q. Cellulase 2. Clostridium acetobutylicum R. Vitamin B12 3. Aspergillus niger S. Butanol 4. Propionibacterium freudenreichii (A) P-4, Q-3, R-1, S-2 (B) P-3, Q-1, R-2, S-4 (C)
