Termination codon is required for stopping high levels of transcription of other regions such as _______ which might interfere with ___________ of the vector
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Termination codon is required for stopping high levels of transcription of other regions such as _______ which might interfere with ___________ of the vector
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Proteins synthesized by carrying out a translation of the vector region and continuing it in the insert region is called as
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Which of the following is present in E. coli rrnB operon for rRNA?
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If an expression vector contains all three, viz. a controllable promoter, a ribosome binding site and an initiation codon
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Translation starts at first _____ codon in the mRNA downstream from the ribosome binding site
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Treatment of reticulocyte cells is done with EGTA. It chelates the calcium ions which are required for functioning of micrococcal nuclease.
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Expression of cloned sequences in E.coli requires a promoter, a ribosome binding site and an initiation codon
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Ribosome binding site in E.coli is composed of which sequence primarily?
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In which of the following systems, transcription and translation are carried out together?
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If overexpression of proteins takes place, they become toxic at times. In such a case _______ colonies are recovered after ______ with the recombinant plasmid.
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In ________ transcription and translation is coupled
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What is the use of adding micrococcal nuclease in the reticulocyte cells?
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