Electrophoresis MCQs

Q. Which of the following statement is true regarding migration of bio molecules? the rate of migration is directly proportional to the current the rate of migration is inversely proportional to current the rate of migration is directly proportional to the resistance of the medium Low voltage is used for the separation of high molecular […]

Q. The technique electrophoresis, for the separation of charged molecules was developed by Tswett Svedberg Tiselius Sanger Answer: Tiselius

Q. In electrophoresis, DNA will migrate towards cathode or positive electrode anode or negative electrode cathode or negative electrode anode or positive electrode Answer: anode or positive electrode

Q. The speed of migration of ions in an electric field depends on magnitude of charge and mass of molecules magnitude of charge and shape of molecules shape and size of the molecule magnitude of charge, shape and mass of molecules Answer: magnitude of charge and shape of molecules

Q. Which of the following will migrate faster? The condition is the molecular weight of the following is equal. Nicked circular DNA Double stranded DNA Single stranded DNA Supercoiled circular DNA Answer: Supercoiled circular DNA

Q. If DNA is digested by endonucleases in four sites giving rise to fragments of which two are equal in length how many bands would be seen after electrophoresis? 3 4 5 Any of the above Answer: 4

Q. What cannot be a reason for using electrophoresis? Comparing two sets of DNA Organizing DNA by shape of backbone Organizing DNA fragments from largest to smallest Organizing DNA in order we can see Answer: Organizing DNA by shape of backbone

Q. What does the electrophoresis apparatus consist of? Gel, buffer chamber and fire pack Buffer chamber and electrophoresis unit Power pack and electrophoresis unit Electrophoresis unit and gel separator Answer: Power pack and electrophoresis unit

Q. Glycerol is added to protein samples before they are loaded to the ‘wells’ of PAGE. The function of glycerol is to _______. Stabilize protein structure Provide density to the sample Helps to bind SDS to the protein Helps to reduce disulfide bonds by β-mercaptoethanol Answer: Provide density to the sample

Q. In SDS-PAGE of protein separation, one SDS molecule will binds to __________. Every amino acid Every two amino acids Every three amino acids Every Four amino acids Answer: Every two amino acids