Culture Media MCQs | Culture Media Multiple Choice Questions and Answers

Questions
1 Which one of the following is true
A Nutrient broth is basal medium
B Addition of selective substances in a solid medium is called enrichment media
C Agar has nutrient properties
D Chocolate medium is selective medium

Answer: Nutrient broth is basal medium
2 Which of the following bacteria has the lowest 50% infective dose (ID50)?
A Shigella sonnei
B Campylobacter jejuni
C Salmonella typhi
D Vibrio cholerae

Answer: Shigella sonnei

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3 The cogulase test is used to differentiate
A Staphylococcus aureus from Staphylococcus epidermidis
B Streptococcus pyogens from Enterococcus faecalis
C Staphylococcus epidermidis from Neisseria meningitidis
D Streptococcus pyogens from Staphylococcus aureus

Answer: Staphylococcus aureus from Staphylococcus epidermidis
4 The identification of bacteria by serologic tests is based on the presence of specific antigens. Which of the following bacterial components is least likely to contain useful antigens?
A Ribosomes
B Cell wall
C Capsule
D Flagella

Answer: Ribosomes
5 Each of the following statements concerning the Gram stain is correct except:
A Mycobacterium tuberculosis stains blue because it has a thick lipid layer
B Escherichia coli stains pink because it has a thin peptidoglycan layer
C Streptococcus pyogens stains blue because it has a thick peptidoglycan layer
D Mycoplasma pneumoniae is not visible in the Gram’s stain because it does not have a cell wall

Answer: Mycobacterium tuberculosis stains blue because it has a thick lipid layer
6 Each of the following organisms is an important cause of urinary tract infections except:
A Bacteriodes fragilis
B Klebsiella pneumoniae
C Proteus mirabilis
D None Of These

Answer: Bacteriodes fragilis
7 A 30 year old woman has non-bloody diarrhea for the past 14 hours. Which one of the to following organisms is least likely to cause this illness?
A Salmonella enteritidis
B Clostridium difficile
C Streptococcus pyogens
D none of the above

Answer: Streptococcus pyogens
8 Each of the following agents is a recognized cause of diarrhea except
A Enterococcus faecalis
B Escherichia coli
C Clostridium perfringens
D Vibrio cholerae

Answer: Enterococcus faecalis
9 Which of the following disease is best diagnosed by serologic means?
A Q Fever
B Actinomycosis
C Pulmonary tuberculosis
D Gonorrhea

Answer: Q Fever
10 In pour-plate method,the medium should be maintained at what temperature?
A 67 degree C
B 37 degree C
C 0 degree C
D 45 degree C

Answer: 45 degree C
11 Which of the following method can be used to determine the number of bacteria quantitatively?
A Pour-plate and spread plate
B Streak-plate
C Spread-plate
D Pour plate

Answer: Pour-plate and spread plate
12 Which of the following are not performed in lyophilization?
A bacterial sample is dehydrated
B cell suspension is frozen at -60 degree to -78 degree C
C vials are connected to high-vacuum line
D agar slant is covered with mineral oil

Answer: agar slant is covered with mineral oil
13 What is the temperature of liquid nitrogen?
A -196 degree C
B -150 degree C
C 0 degree C
D None Of The Above

Answer: -196 degree C
14 Which of the following is a characteristic of beef extract?
A aqueous extract of lean beef tissue
B aqueous extract of yeast cells
C complex carbohydrate obtained from certain marine algae
D product resulting from the digestion of proteinaceous materials

Answer: aqueous extract of lean beef tissue
15 Which of the following is a rich source of B vitamins?
A Yeast extract
B Beef extract
C Peptone
D Agar

Answer: Yeast extract
16 Nutrient broth, a liquid media contains beef extract and peptone respectively in how much amounts?
A 0.3%, 0.5%
B 0.7%, 0.3%
C 0.2%, 0.4%
D 0.1%, 0.6%

Answer: 0.3%, 0.5%
17 Which of the following is a Complex media for fungal growth?
A Potato Dextrose Agar(PDA) media
B Mac Conkey Agar media
C Nutrient broth
D Luria-Bertani media

Answer: Potato Dextrose Agar(PDA) media
18 Which of the following are functions of Maintenance Media?
A used for the maintenance of the viability and physiological characteristics
B used for determining the type of growth produced by bacteria
C used for assay of vitamins,amino acids
D used for determining the bacterial content

Answer: used for the maintenance of the viability and physiological characteristics
19 Which of the following bacteria requires nicotinic acid as a growth factor in their media?
A Nitrosomonas sp
B E. coli
C Proteus vulgaris
D none of the above

Answer: Proteus vulgaris
20 Which of the following instrument is used for sterilizing the media after it has been prepared?
A Incubator
B Inoculum Needle
C Laminar Air Flow Chamber
D Autoclave

Answer: Autoclave
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