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Genetic Engineering Quiz | Genetic Engineering Objective Type Questions and Answers

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Questions
21 A vector should have which of the following properties i) MCS ii) Small size iii) Multiple ori iv)low replication speed
A i, ii, iii
B ii, iii, iv
C i, ii, iv
D i, iii, iv

Answer: i, ii, iv
22 The chromogenic substrate of X-gal is:
A 5-chloro-4-bromo-3indolyl-beta-D-galactoside
B 5-bromo-4-chloro-3indolyl-beta-D-galactoside
C 5-chloro-5-bromo-3indolyl-beta-D-galactoside
D 5-bromo-5-chloro-3indolyl-beta-D-galactoside

Answer: 5-bromo-4-chloro-3indolyl-beta-D-galactoside
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23 Which of the following molecules is useful as a measure of relatedness beyond species level between organisms:
A mRNA
B tRNA
C rRNA
D miRNA

Answer: rRNA
24 Indole -3-acetic acid is:
A A gibberellin
B A cytokinin
C An auxin
D All of these

Answer: An auxin
25 Hairy root cultures are produced by using:
A Agrobacterium tumefaciens
B Agrobacterium rhizogenes
C Agrobacterium vitis
D All of these

Answer: Agrobacterium rhizogenes
26 Polyhedrin protein is associated with one of the following viruses:
A HIV
B HSV
C NPV
D None Of The Above

Answer: NPV
7 Which of the following is used as a fusogen:
A Polyethylene glycol
B CaMV
C Sendai Virus
D None Of The Above

Answer: Polyethylene glycol
28 Restriction endonucleases are usually supplied in:
A 10% glycerol
B 30% glycerol
C 50% glycerol
D 80% glycerol

Answer: 50% glycerol
29 Choose the incorrect statement for second DNA strand synthesis.
A The RNA: DNA complex is treated with the enzyme RNaseH and DNA pol
B The enzyme RNaseH is responsible for nicking the RNA strand and leaving free 3’ hydroxyl ends
C These RNA fragments can be used as primers
D There is no portion of RNA left attached to the DNA strand

Answer: There is no portion of RNA left attached to the DNA strand
30 What would not happen if the RNA strand is completely removed from RNA: DNA hybrid?
A There are no chances of the synthesis of the second DNA strand
B Chance complementarity would take place
C Hairpin structure would be formed
D Hairpin structure is formed is not the final structure

Answer: There are no chances of the synthesis of the second DNA strand
31 Choose the correct statement with respect to the self priming method of cDNA synthesis.
A It is less preferred than RNaseH method
B A hairpin structure is formed with guarantee
C The sequence corresponding to the 5’ end is lost
D Reverse transcriptase is not used

Answer: The sequence corresponding to the 5’ end is lost
32 Choose the incorrect statement for the method homopolymer tailing.
A The first step is the RNA: DNA hybrid synthesis
B Terminal transferase is used for the addition of nucleotides on 3’ end
C Terminal transferase adds only at DNA strands
D None of the above

Answer: Terminal transferase adds only at DNA strands
33 Choose the correct statement for RACE.
A It stands for Random Amplification of cDNA ends
B It is for cloning particular cDNA ends
C It is only of one type, which is 5’ RACE
D Sequence data is not available in any case

Answer: It is only of one type, which is 5’ RACE

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