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Sterilization MCQs | Sterilization Multiple Choice Questions and Answers

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Questions
1 The process in which all living cells, spores and viruses are completely destroyed from an object is called _____.
A Disinfection
B Pasteurization
C Sterilization
D Antisepsis

Answer: Sterilization
2 _______ is an antiseptic.
A Chlorine
B Copper Sulphate
C Ozone
D 70% ethanol

Answer: 70% ethanol
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3 Same DNA sequence may code for more than one protein by ______
A Gene splitting
B RNA splitting
C Alternative splicing of RNA
D None

Answer: Alternative splicing of RNA
4 Glucose that is taken in by a bacterial cell is made to stay within the cell by _______.
A Attaching to membranes
B Immediate utilization
C Phosphorylation
D None of the above

Answer: Phosphorylation
5 _____ is a predator bacterium that infects other gram negative bacteria.
A Haemophilus influenza
B Pseudomonas putida
C Proteus mirabilis
D Bdellovibrio

Answer: Bdellovibrio
6 Which of the following is not a type of sterilization?
A Batch
B Continuous
C Filter
D Submerged

Answer: Submerged
7 What do you mean by sterilization?
A Purification of products
B Recovery of products
C Elimination of contamination
D None Of The Above

Answer: Elimination of contamination
8 The highest feasible temperature for batch sterilization is ________
A 124°C
B 120°C
C 121°C
D None of these

Answer: 121°C
9 The destruction of microorganisms by moist heat is described by ______________
A Zero-order reaction
B First-order reaction
C Third-order reaction
D Second-order reaction

Answer: First-order reaction
10 The long exposure of batch sterilization may lead to ____________
A Purification of media
B Recovery of media
C Degradation
D Good quality of product

Answer: Degradation
11 What is the advantage of batch sterilization over continuous sterilization?
A Superior maintenance of medium quality
B Ease of scale-up
C Automatic control
D Lower equipment costs

Answer: Lower equipment costs
12 The equation for Del factor is _____________
A ∇ = ln (N0/Nt)
B ∇ = ln (Nt/N0)
C ∇ = ln (N0)
D None of the above

Answer: ∇ = ln (N0/Nt)
13 The Del Factor decreases as the initial number of organisms _____________
A Decreases
B Increases
C Remains constant
D Becomes zero

Answer: Decreases
14 How long does it take for the autoclave to complete its cycle?
A 30-35 minutes
B 50 min to 1 hr
C 15-20 minutes
D 10-15 minutes

Answer: 15-20 minutes
15 Which scientists introduced the term ‘Del factor’?
A Deindoerfer and Humphrey
B Jacob and Monod
C Banks and Corbett
D None of these

Answer: Deindoerfer and Humphrey
16 Which of the following reaction occurs during the sterilization which results in browning of media?
A Sandmeyer reaction
B Maillard reaction
C Cannizzaro reaction
D Gattermann reaction

Answer: Maillard reaction
17 If the foreign microorganism invades the fermentation, which of the following is not likely to occur?
A The medium will not allow the growth of contaminant
B The contaminant may outgrow the production organism
C The contaminant may contaminate the final product
D The contaminant may degrade the final product

Answer: The medium will not allow the growth of contaminant
18 UHT sterilization involves high temperature exposure of objects for _____.
A 1 – 3 seconds
B 1 – 3 minutes
C 1 – 3 hours
D 10 – 30 hours

Answer: 1 – 3 seconds
19 _____ RNA polymerase has a single subunit.
A E. coli
B Bacillus subtilis
C T7
D λ phage

Answer: T7
20 The best form of DNA to transform E. coli is ______.
A Linear
B Covalently closed
C Nicked circles
D None Of The Above

Answer: Covalently closed
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