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Tissue Culture Quiz | Tissue Culture Multiple Choice Questions(MCQs) and Answers

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Questions
1 Growth hormone producing apical dominance is
A Auxin
B Gibberellin
C Ethylene
D Cytokinin

Answer: Auxin
2 Part of plant used for culturing is called
A Scion
B Explant
C Stock
D Callus

Answer: Explant
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3 Callus is
A Tissue that forms embryo
B An insoluble carbohydrate
C Tissue that grows to form embryoid
D Un organised actively dividing mass of cells maintained in cultured

Answer: Un organised actively dividing mass of cells maintained in cultured
4 Cybrids are produced by
A Fusion of two different nuclei from two different species
B Fusion of two same nuclei from same species
C Nucleus of one species but cytoplasm from both the parent species
D None of the above

Answer: Nucleus of one species but cytoplasm from both the parent species
5 The most widely used chemical for protoplast fusion, as fusogens, is
A Manitol
B Sorbitol
C Mannol
D Poly ethylene glycol (PEG)

Answer: Poly ethylene glycol (PEG)
6 DMSO (Dimethyl sulfoxide) is used as
A Gelling agent
B alkaylating agent
C Chelating agent
D Cryoprotectant

Answer: Cryoprotectant
7 Hormone pair required for a callus to differentiate are
A auxin and cytokinin
B auxin and ethylene
C auxin and absiccic acid
D cytokinins and gibberllin

Answer: auxin and cytokinin
8 Synthetic seed is produced by encapsulating somatic embryo with
A sodium chloride
B sodium alginate
C sodium acetate
D sodium nitrate

Answer: sodium alginate
9 The production of secondary metabolites require the use of
A protoplast
B cell suspension
C meristem
D auxillary buds

Answer: cell suspension
10 Who is the father of tissue culture?
A Bonner
B Haberlandt
C Laibach
D Gautheret

Answer: Haberlandt
11 Which vector is mostly used in crop improvement?
A Plasmid
B Cosmid
C Phasmid
D Agrobacterium

Answer: Agrobacterium
12 Which of the following plant cell will show totipotency?
A Xylem vessels
B Sieve tube
C Meristem
D Cork cells

Answer: Meristem
13 Somaclonal variations are the ones
A Caused by mutagens
B Produce during tissue culture
C Caused by gamma rays
D Induced during sexual embryogeny

Answer: Produce during tissue culture
14 To obtain haploid plant, we culture
A Entire anther
B Nucleus
C Embryo
D Apical bud

Answer: Entire anther
15 A medium which is composed of chemically defined compound is called
A Natural media
B Synthetic media
C Artificial media
D None of these

Answer: Synthetic media
16 The growth of plant tissues in artificial media is called___________
A Gene expression
B Transgenesis
C Plant tissue culture
D Cell hybridization

Answer: Plant tissue culture
17 Which of the following is NOT the feature of plant cells?
A Presence of centrioles
B The cell wall outside the cell membrane
C Cell-cell communication through plasmodesmata
D Consists of plastids

Answer: Presence of centrioles
18 Name the term given to the ability of single cells to divide and produce all the differentiated cell in the organism?
A Totipotency
B Multipotent
C Unipotent
D Pluripotent

Answer: Totipotency
19 Out of the following, which one is NOT the basic component of culture media used for plant cultivation?
A Complex mixture of salts
B Amino acids
C Serum albumin
D Sugar/ sucrose

Answer: Serum albumin
20 Mark the INCORRECT statement about agar, a gelling agent in plant tissue culture medium?
A Not digested by plant enzymes
B It does not use in microprapogation work
C It does not react with media constituents
D Remain stable at incubation temperature

Answer: It does not use in microprapogation work
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