Download PDF
Free download in PDF Electrophoresis Multiple Choice Questions and Answers for competitive exams. These short objective type questions with answers are very important for competitive exams like IIT-JEE, AIIMS etc. as well as Board exams. These short solved questions or quizzes are provided by Gkseries.
(1)
Which technique separates charged particles using electric field?
[A]
Hydrolysis
[B]
Electrophoresis
[C]
Protein synthesis
[D]
Protein denaturing
(2)
The speed of migration of ions in electric field depends upon:
[A]
Shape and size of molecule
[B]
Magnitude of charge and shape of molecule
[C]
Magnitude of charge shape and mass of molecule
[D]
Magnitude of charge and mass of molecule
Answer: Magnitude of charge and shape of molecule
(3)
Which of the following statements is true about migration of biomolecules?
[A]
The rate of migration is directly proportional to the resistance of medium
[B]
Rate of migration is directly proportional to current
[C]
Low voltage is used for separation of high mass molecules
[D]
Rate of migration is inversely proportional to current
Answer: Rate of migration is directly proportional to current
(4)
Agarose can be extracted from which of the following?
[A]
Lycazusican esculentum
[B]
Ficum benghalensis
[C]
Gracilaria esculentus
[D]
Agrostis stolonifera
Answer: Gracilaria esculentus
(5)
The electrophoretic mobility denoted as µ is mathematically expressed as:
[A]
VE
[B]
V/E
[C]
E/V
[D]
1/EV
(6)
Which of the following factors does not influence electrophoretic mobility?
[A]
Molecular weight
[B]
Shape of molecule
[C]
Size of molecule
[D]
Stereochemistry of molecule
Answer: Stereochemistry of molecule
(7)
When is electrophoresis not used?
[A]
Separation of proteins
[B]
Separation of amino acids
[C]
Separation of Lipids
[D]
Separation of nucleic acids
Answer: Separation of Lipids
(8)
Which of the following cannot be used for the separation of nucleic acids?
[A]
SDS – PAGE
[B]
PAGE
[C]
Northern blotting
[D]
PAGE
(9)
The polymerization of the gel used in PAGE occurs between polyacrylamide and ____________
[A]
N, N – acrylamide
[B]
Bisacrylamide
[C]
N – methyleneacrylamide
[D]
N, N – methylene bisacrylamide
Answer: N, N – methylene bisacrylamide
(10)
The fluorescent dye such Ethidium is used for visualizing DNA. How do ethidium binds to DNA?
[A]
Stacked between histone molecules
[B]
Binds to the nucleotide base
[C]
Intercalated between the stacked bases
[D]
Binds to the phosphodiester backbone
Answer: Intercalated between the stacked bases
(11)
Pulse field gel electrophoresis separates DNA molecules of size ___________
[A]
10 – 20 bp
[B]
20 – 30 Kb
[C]
30 – 50 Kb
[D]
40 – 50 bp
(12)
Pulse field gel electrophoresis was developed by ____________
[A]
Collins and John
[B]
Kary Mullis
[C]
Patrick O’ Farrell
[D]
Schwartz and Cantor
Answer: Schwartz and Cantor
(13)
When voltage ‘V’ is applied across a pair of electrode (cathode and anode), a potential gradient ‘E’ is created between the electrodes. We can calculate ‘E’ as:
[A]
E = V/d
[B]
E = (1/V) x q
[C]
E = (Vd)/q
[D]
None of these
(14)
For the separation of DNA by electrophoresis, which of the following method is commonly used?
[A]
Agarose – vertical
[B]
Agarose – horizontal
[C]
PAGE – vertical
[D]
PAGE – horizontal
Answer: Agarose – horizontal
(15)
Sodium dodecyl sulfate (SDS) used in SDS PAGE is___________.
[A]
An anionic detergent
[B]
A cationic detergent
[C]
A non-ionic detergent
[D]
An anion exchanger
Answer: An anionic detergent
(16)
In SDS-PAGE, migration of protein is effected by ____________.
[A]
Charge of protein
[B]
Size of protein
[C]
Net charge of protein
[D]
All of these
(17)
The main advantage of discontinuous buffer system in SDS and Native PAGE is ________.
[A]
Conformation of protein is conserved
[B]
Constantly maintain the charge of proteins
[C]
Assist in migration of protein
[D]
Enhance resolution of separation
Answer: Enhance resolution of separation
(18)
The pH of (i) stacking, (ii) resolving gel and (iii) tank buffer in SDS PAGE is _________ respectively.
[A]
(i). 8.30 (ii) 8.80 (iii) 6.80
[B]
(i). 6.80 (ii) 8.80 (iii) 8.30
[C]
(i). 8.30 (ii) 6.80 (iii) 8.80
[D]
Any of the above
Answer: (i). 6.80 (ii) 8.80 (iii) 8.30
(19)
The role of APS in SDS PAGE is to________.
[A]
Act as a catalyst in the polymerization of acrylamide
[B]
Act as a source of free radicals
[C]
Act as a bridge between acrylamide and bis-acrylamide
[D]
Act as a pore builder in the polymerized gel
Answer: Act as a source of free radicals
(20)
The ‘tracking dye’ used in SDS PAGE will be ______.
[A]
Anionic
[B]
Cationic
[C]
Non-ionic
[D]
Amphipathic
Take Mock Tests