Electrophoresis MCQs | Electrophoresis Multiple Choice Questions and Answers
Questions
1
Which technique separates charged particles using electric field?
Answer: Electrophoresis
2
The speed of migration of ions in electric field depends upon:
Answer: Magnitude of charge and shape of molecule
3
Which of the following statements is true about migration of biomolecules?
Answer: Rate of migration is directly proportional to current
4
Agarose can be extracted from which of the following?
Answer: Gracilaria esculentus
5
The electrophoretic mobility denoted as µ is mathematically expressed as:
Answer: V/E
6
Which of the following factors does not influence electrophoretic mobility?
Answer: Stereochemistry of molecule
8
Which of the following cannot be used for the separation of nucleic acids?
Answer: SDS – PAGE
9
The polymerization of the gel used in PAGE occurs between polyacrylamide and ____________
Answer: N, N – methylene bisacrylamide
10
The fluorescent dye such Ethidium is used for visualizing DNA. How do ethidium binds to DNA?
Answer: Intercalated between the stacked bases
11
Pulse field gel electrophoresis separates DNA molecules of size ___________
Answer: 30 – 50 Kb
12
Pulse field gel electrophoresis was developed by ____________
Answer: Schwartz and Cantor
13
When voltage ‘V’ is applied across a pair of electrode (cathode and anode), a potential gradient ‘E’ is created between the electrodes. We can calculate ‘E’ as:
Answer: E = V/d
14
For the separation of DNA by electrophoresis, which of the following method is commonly used?
Answer: Agarose – horizontal
15
Sodium dodecyl sulfate (SDS) used in SDS PAGE is___________.
Answer: An anionic detergent
16
In SDS-PAGE, migration of protein is effected by ____________.
Answer: Size of protein
17
The main advantage of discontinuous buffer system in SDS and Native PAGE is ________.
Answer: Enhance resolution of separation
18
The pH of (i) stacking, (ii) resolving gel and (iii) tank buffer in SDS PAGE is _________ respectively.
Answer: (i). 6.80 (ii) 8.80 (iii) 8.30
19
The role of APS in SDS PAGE is to________.
Answer: Act as a source of free radicals
