Download PDF
Free download in PDF Genetic Engineering Objective Type Questions and Answers for competitive exams. These short objective type questions with answers are very important for Board exams as well as competitive exams. These short solved questions or quizzes are provided by Gkseries.
(21)
A vector should have which of the following properties
i) MCS ii) Small size iii) Multiple ori iv)low replication speed
[A]
i, ii, iii
[B]
ii, iii, iv
[C]
i, ii, iv
[D]
i, iii, iv
(22)
The chromogenic substrate of X-gal is:
[A]
5-chloro-4-bromo-3indolyl-beta-D-galactoside
[B]
5-bromo-4-chloro-3indolyl-beta-D-galactoside
[C]
5-chloro-5-bromo-3indolyl-beta-D-galactoside
[D]
5-bromo-5-chloro-3indolyl-beta-D-galactoside
Answer: 5-bromo-4-chloro-3indolyl-beta-D-galactoside
(23)
Which of the following molecules is useful as a measure of relatedness beyond species level between organisms:
[A]
mRNA
[B]
tRNA
[C]
rRNA
[D]
miRNA
(24)
Indole -3-acetic acid is:
[A]
A gibberellin
[B]
A cytokinin
[C]
An auxin
[D]
All of these
(25)
Hairy root cultures are produced by using:
[A]
Agrobacterium tumefaciens
[B]
Agrobacterium rhizogenes
[C]
Agrobacterium vitis
[D]
All of these
Answer: Agrobacterium rhizogenes
(26)
Polyhedrin protein is associated with one of the following viruses:
[A]
HIV
[B]
HSV
[C]
NPV
[D]
None Of The Above
(7)
Which of the following is used as a fusogen:
[A]
Polyethylene glycol
[B]
CaMV
[C]
Sendai Virus
[D]
None Of The Above
Answer: Polyethylene glycol
(28)
Restriction endonucleases are usually supplied in:
[A]
10% glycerol
[B]
30% glycerol
[C]
50% glycerol
[D]
80% glycerol
(29)
Choose the incorrect statement for second DNA strand synthesis.
[A]
The RNA: DNA complex is treated with the enzyme RNaseH and DNA pol
[B]
The enzyme RNaseH is responsible for nicking the RNA strand and leaving free 3’ hydroxyl ends
[C]
These RNA fragments can be used as primers
[D]
There is no portion of RNA left attached to the DNA strand
Answer: There is no portion of RNA left attached to the DNA strand
(30)
What would not happen if the RNA strand is completely removed from RNA: DNA hybrid?
[A]
There are no chances of the synthesis of the second DNA strand
[B]
Chance complementarity would take place
[C]
Hairpin structure would be formed
[D]
Hairpin structure is formed is not the final structure
Answer: There are no chances of the synthesis of the second DNA strand
31
Choose the correct statement with respect to the self priming method of cDNA synthesis.
[A]
It is less preferred than RNaseH method
[B]
A hairpin structure is formed with guarantee
[C]
The sequence corresponding to the 5’ end is lost
[D]
Reverse transcriptase is not used
Answer: The sequence corresponding to the 5’ end is lost
32
Choose the incorrect statement for the method homopolymer tailing.
[A]
The first step is the RNA: DNA hybrid synthesis
[B]
Terminal transferase is used for the addition of nucleotides on 3’ end
[C]
Terminal transferase adds only at DNA strands
[D]
None of the above
Answer: Terminal transferase adds only at DNA strands
33
Choose the correct statement for RACE.
[A]
It stands for Random Amplification of cDNA ends
[B]
It is for cloning particular cDNA ends
[C]
It is only of one type, which is 5’ RACE
[D]
Sequence data is not available in any case
Answer: It is only of one type, which is 5’ RACE
Take Mock Tests