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Free download in PDF Principles and Processes of Biotechnology Multiple Choice Questions with Answers for competitive exams. These short objective type questions with answers are very important for Board exams as well as competitive exams like NEET, AIIMS, JIPMER etc. These short solved questions or quizzes are provided by Gkseries./p>
(1)
One of the key factors which makes the plasmid the vector in genetic engineering is that
[A]
It is resistant to antibiotics
[B]
It is resistant to restriction enzymes
[C]
Its ability to carry a foreign gene
[D]
Its ability to cause infection in the host
Answer: Its ability to carry a foreign gene
(2)
Which of the following is used as a best genetic vector in plants
[A]
Bacillus thurienglnesis
[B]
Agrobacterium thumifaciens
[C]
Psendomonas putida
[D]
All of the above
Answer: Agrobacterium thumifaciens
(3)
The polymerase chain reaction is a technique that
[A]
It is used for in vivo replication of DNA
[B]
It is used for in vivo synthesis of mRNA
[C]
It is used for in vitro synthesis of mRNA
[D]
It is used for in vitro replication of specific DNA sequence using thermostable DNA polymerase.
Answer: It is used for in vitro replication of specific DNA sequence using thermostable DNA polymerase.
(4)
The construction of the first recombinant DNA was done by using the native plasmid of
[A]
E coli
[B]
Salmonella typhimurium
[C]
Bacillus thuringiensis
[D]
Agrobacterium
Answer: Salmonella typhimurium
(5)
Gel electrophoresis is used for
[A]
Construction of recombinant DNA by joining with cloning vectors
[B]
Isolation of DNA molecules
[C]
Cutting of DNA is to fragments
[D]
Separation of DNA fragments according to their size
Answer: Separation of DNA fragments according to their size
(6)
Significant of ‘heat shoch’ method in bacterial transformation is to facilitate ?
[A]
Binding of DNA to the cell wall
[B]
Update of DNA through membrane transport proteins
[C]
Update of DNA through transient pores in the bacterial cell wall
[D]
Expression of antibiotic resistant gene
Answer: Update of DNA through transient pores in the bacterial cell wall
(7)
Which of the following palindromic bare sequences in DNA can be easily cut at about the middle by some particular restriction enzyme.
[A]
5’CACGTA 3’ : 3’CTCAGT 5’
[B]
5’CGTTCG 3’ : 3’ATGGTA 5’
[C]
5’GATATC 3’ : 3’CTACTA 5’
[D]
5’GAATTC 3’ : CTTAAG 5’
Answer: 5’GAATTC 3’ : CTTAAG 5’
(8)
Agarose extracted from sea weeds in used in
[A]
Spectrophotometry
[B]
Tissue culture
[C]
PCR
[D]
Gel electrophoresis
Answer: Gel electrophoresis
(9)
Which one of the following technique made it possible to genetically engineered living organisms ?
[A]
Recombinant DNA technique
[B]
X-ray diffraction
[C]
Heavier isotope labelling
[D]
Hybridization
Answer: Recombinant DNA technique
(10)
There is a restriction endonucleare called EcoRI. What does ‘co’ part in it stand for ?
[A]
Colon
[B]
Coelom
[C]
Coenzyme
[D]
Coli
(11)
A single stand of nucleic acid tagged tagged with a radioactive molecule is called
[A]
Vector
[B]
Selectable marker
[C]
Plasmid
[D]
Probe
(12)
For transformation, micro particles coated with DNA to be bombared with gene gun are made up of
[A]
Silver or Platinum
[B]
Platinum or Zine
[C]
Silicon or Platinum
[D]
Gold or Tungsten
(13)
Biolistics (Gene-gun) is suitable for
[A]
Disarming pathogen vectors
[B]
Transformation of plant cells
[C]
Constructing recombinant DNA by joining with vectors
[D]
DNA fingerprinting
Answer: Transformation of plant cells
(14)
In genetic engineering, the antibiotics are used
[A]
As selectable markers
[B]
To select healthy vectors
[C]
As sequence from where replication starts
[D]
To keep the culture free from infection
Answer: As selectable markers
(15)
Restriction enzymes were discovered by
[A]
Smith and Nathans
[B]
Alexander Fleming
[C]
Berg
[D]
None
Answer: Smith and Nathans
(16)
Bacteria protect themselves from viruses by fragmenting viral DNA with
[A]
Ligase
[B]
Endonuclease
[C]
Exonuclease
[D]
Gyrase
(17)
Klenow fragment is derived from
[A]
DNA Ligase
[B]
DNA Pol-I
[C]
DNA Pol-II
[D]
Reverse Transcriptase
(18)
Southern blotting is
[A]
Attachment of probes to DNA fragments
[B]
Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet
[C]
Comparison of DNA fragments to two sources
[D]
Transfer of DNA fragments to electrophoretic gel from cellulose membrane
Answer: Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet
(19)
ELISA is
[A]
Using radiolabelled second antibody
[B]
Usage of RBCs
[C]
Using complement-mediated cell lysis
[D]
Addition of substrate that is converted into a coloured end product
Answer: Addition of substrate that is converted into a coloured end product
(20)
The Golden Rice variety is rich in
[A]
Vitamin C
[B]
Β-carotene and ferritin
[C]
Biotin
[D]
Lysine
Answer: Β-carotene and ferritin
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