Principles and Processes of Biotechnology Multiple Choice Questions with Answers | Principles and Processes of Biotechnology Quiz

Questions

1 One of the key factors which makes the plasmid the vector in genetic engineering is that

A It is resistant to antibiotics

B It is resistant to restriction enzymes

C Its ability to carry a foreign gene

D Its ability to cause infection in the host

View Answer

Answer:Its ability to carry a foreign gene

2 Which of the following is used as a best genetic vector in plants

A Bacillus thurienglnesis

B Agrobacterium thumifaciens

C Psendomonas putida

D All of the above

View Answer

Answer: Agrobacterium thumifaciens

3 The polymerase chain reaction is a technique that

A It is used for in vivo replication of DNA

B It is used for in vivo synthesis of mRNA

C It is used for in vitro synthesis of mRNA

D It is used for in vitro replication of specific DNA sequence using thermostable DNA polymerase.

View Answer

Answer:It is used for in vitro replication of specific DNA sequence using thermostable DNA polymerase.

4 The construction of the first recombinant DNA was done by using the native plasmid of

A E coli

B Salmonella typhimurium

C Bacillus thuringiensis

D Agrobacterium

View Answer

Answer: Salmonella typhimurium

5 Gel electrophoresis is used for

A Construction of recombinant DNA by joining with cloning vectors

B Isolation of DNA molecules

C Cutting of DNA is to fragments

D Separation of DNA fragments according to their size

View Answer

Answer: Separation of DNA fragments according to their size

6 Significant of ‘heat shoch’ method in bacterial transformation is to facilitate ?

A Binding of DNA to the cell wall

B Update of DNA through membrane transport proteins

C Update of DNA through transient pores in the bacterial cell wall

D Expression of antibiotic resistant gene

View Answer

Answer: Update of DNA through transient pores in the bacterial cell wall

7 Which of the following palindromic bare sequences in DNA can be easily cut at about the middle by some particular restriction enzyme.

A 5’CACGTA 3’ : 3’CTCAGT 5’

B 5’CGTTCG 3’ : 3’ATGGTA 5’

C 5’GATATC 3’ : 3’CTACTA 5’

D 5’GAATTC 3’ : CTTAAG 5’

View Answer

Answer: 5’GAATTC 3’ : CTTAAG 5’

8 Agarose extracted from sea weeds in used in

A Spectrophotometry

B Tissue culture

C PCR

D Gel electrophoresis

View Answer

Answer:Gel electrophoresis

9 Which one of the following technique made it possible to genetically engineered living organisms ?

A Recombinant DNA technique

B X-ray diffraction

C Heavier isotope labelling

D Hybridization

View Answer

Answer: Recombinant DNA technique

10 There is a restriction endonucleare called EcoRI. What does ‘co’ part in it stand for ?

A Colon

B Coelom

C Coenzyme

D Coli

View Answer

Answer: Coli

11 A single stand of nucleic acid tagged tagged with a radioactive molecule is called

A Vector

B Selectable marker

C Plasmid

D Probe

View Answer

Answer: Probe

12 For transformation, micro particles coated with DNA to be bombared with gene gun are made up of

A Silver or Platinum

B Platinum or Zine

C Silicon or Platinum

D Gold or Tungsten

View Answer

Answer: Gold or Tungsten

13 Biolistics (Gene-gun) is suitable for

A Disarming pathogen vectors

B Transformation of plant cells

C Constructing recombinant DNA by joining with vectors

D DNA fingerprinting

View Answer

Answer: Transformation of plant cells

14 In genetic engineering, the antibiotics are used

A As selectable markers

B To select healthy vectors

C As sequence from where replication starts

D To keep the culture free from infection

View Answer

Answer: As selectable markers

15 Restriction enzymes were discovered by

A Smith and Nathans

B Alexander Fleming

C Berg

D None

View Answer

Answer: Smith and Nathans

16 Bacteria protect themselves from viruses by fragmenting viral DNA with

A Ligase

B Endonuclease

C Exonuclease

D Gyrase

View Answer

Answer:Endonuclease

17 Klenow fragment is derived from

A DNA Ligase

B DNA Pol-I

C DNA Pol-II

D Reverse Transcriptase

View Answer

Answer: DNA Pol-I

18 Southern blotting is

A Attachment of probes to DNA fragments

B Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet

C Comparison of DNA fragments to two sources

D Transfer of DNA fragments to electrophoretic gel from cellulose membrane

View Answer

Answer: Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet

19 ELISA is

A Using radiolabelled second antibody

B Usage of RBCs

C Using complement-mediated cell lysis

D Addition of substrate that is converted into a coloured end product

View Answer

Answer: Addition of substrate that is converted into a coloured end product

20 The Golden Rice variety is rich in

A Vitamin C

B Β-carotene and ferritin

C Biotin

D Lysine

View Answer

Answer:Β-carotene and ferritin

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