Tissue Culture Quiz | Tissue Culture Multiple Choice Questions(MCQs) and Answers

(1) Growth hormone producing apical dominance is
[A] Auxin
[B] Gibberellin
[C] Ethylene
[D] Cytokinin
Answer: Auxin
(2) Part of plant used for culturing is called
[A] Scion
[B] Explant
[C] Stock
[D] Callus
Answer: Explant

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(3) Callus is
[A] Tissue that forms embryo
[B] An insoluble carbohydrate
[C] Tissue that grows to form embryoid
[D] Un organised actively dividing mass of cells maintained in cultured
Answer: Un organised actively dividing mass of cells maintained in cultured
(4) Cybrids are produced by
[A] Fusion of two different nuclei from two different species
[B] Fusion of two same nuclei from same species
[C] Nucleus of one species but cytoplasm from both the parent species
[D] None of the above
Answer: Nucleus of one species but cytoplasm from both the parent species
(5) The most widely used chemical for protoplast fusion, as fusogens, is
[A] Manitol
[B] Sorbitol
[C] Mannol
[D] Poly ethylene glycol (PEG)
Answer: Poly ethylene glycol (PEG)
(6) DMSO (Dimethyl sulfoxide) is used as
[A] Gelling agent
[B] alkaylating agent
[C] Chelating agent
[D] Cryoprotectant
Answer: Cryoprotectant
(7) Hormone pair required for a callus to differentiate are
[A] auxin and cytokinin
[B] auxin and ethylene
[C] auxin and absiccic acid
[D] cytokinins and gibberllin
Answer: auxin and cytokinin
(8) Synthetic seed is produced by encapsulating somatic embryo with
[A] sodium chloride
[B] sodium alginate
[C] sodium acetate
[D] sodium nitrate
Answer: sodium alginate
(9) The production of secondary metabolites require the use of
[A] protoplast
[B] cell suspension
[C] meristem
[D] auxillary buds
Answer: cell suspension
(10) Who is the father of tissue culture?
[A] Bonner
[B] Haberlandt
[C] Laibach
[D] Gautheret
Answer: Haberlandt
(11) Which vector is mostly used in crop improvement?
[A] Plasmid
[B] Cosmid
[C] Phasmid
[D] Agrobacterium
Answer: Agrobacterium
(12) Which of the following plant cell will show totipotency?
[A] Xylem vessels
[B] Sieve tube
[C] Meristem
[D] Cork cells
Answer: Meristem
(13) Somaclonal variations are the ones
[A] Caused by mutagens
[B] Produce during tissue culture
[C] Caused by gamma rays
[D] Induced during sexual embryogeny
Answer: Produce during tissue culture
(14) To obtain haploid plant, we culture
[A] Entire anther
[B] Nucleus
[C] Embryo
[D] Apical bud
Answer: Entire anther
(15) A medium which is composed of chemically defined compound is called
[A] Natural media
[B] Synthetic media
[C] Artificial media
[D] None of these
Answer: Synthetic media
(16) The growth of plant tissues in artificial media is called___________
[A] Gene expression
[B] Transgenesis
[C] Plant tissue culture
[D] Cell hybridization
Answer: Plant tissue culture
(17) Which of the following is NOT the feature of plant cells?
[A] Presence of centrioles
[B] The cell wall outside the cell membrane
[C] Cell-cell communication through plasmodesmata
[D] Consists of plastids
Answer: Presence of centrioles
(18) Name the term given to the ability of single cells to divide and produce all the differentiated cell in the organism?
[A] Totipotency
[B] Multipotent
[C] Unipotent
[D] Pluripotent
Answer: Totipotency
(19) Out of the following, which one is NOT the basic component of culture media used for plant cultivation?
[A] Complex mixture of salts
[B] Amino acids
[C] Serum albumin
[D] Sugar/ sucrose
Answer: Serum albumin
(20) Mark the INCORRECT statement about agar, a gelling agent in plant tissue culture medium?
[A] Not digested by plant enzymes
[B] It does not use in microprapogation work
[C] It does not react with media constituents
[D] Remain stable at incubation temperature
Answer: It does not use in microprapogation work

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