Q. Glycerol is added to protein samples before they are loaded to the ‘wells’ of PAGE. The function of glycerol is to _______. Stabilize protein structure Provide density to the sample Helps to bind SDS to the protein Helps to reduce disulfide bonds by β-mercaptoethanol Answer: Provide density to the sample
Q. In SDS-PAGE of protein separation, one SDS molecule will binds to __________. Every amino acid Every two amino acids Every three amino acids Every Four amino acids Answer: Every two amino acids
In SDS-PAGE of protein separation, one SDS molecule will binds to Read More »
Q. Electrophoresis is not used for the separation of ________. Nucleic acids Proteins Amino acids Lipids Answer: Lipids
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Q. The ratio of velocity (‘v’) of biomolecule in a medium under constant electric field (‘E’) is called ‘Electrophoretic mobility’ denoted as ‘µ’. ‘µ’ is mathematically expressed as: µ = E/v µ = 1/(Ev) µ = VE µ = v/E Answer: µ = v/E
Q. Function of β-mercaptoethanol in SDS-PAGE is__________. To give negative charges to amino acids in the proteins For the oxidation of disulfide bonds in the proteins For breaking hydrogen bonds in the proteins For the reduction of disulfide bonds in the proteins Answer: For the reduction of disulfide bonds in the proteins
Q. In electrophoresis, the electrophoretic mobility (‘µ’) is determines the characteristics of migration of different biomolecules. Which of the following is not having any influence in ‘µ’? Size of molecule Shape of molecule Molecular weight Stereochemistry of molecule Answer: Stereochemistry of molecule
Q. In native-PAGE the separation of protein is influenced by ___________. Charge of protein Size of protein pI of protein Both (a) and (b) Answer: Both (a) and (b)
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Q. The role of urea in PAGE separation of DNA is to _______. Act as anion Act as cation Helps to denature the DNA Provide buffer stability of the gel Answer: Helps to denature the DNA
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Q. In a native PAGE, proteins are separated on the basis of net negative charge net charge and size net positive charges size net positive charge Answer: net charge and size
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Q. In an SDS-PAGE proteins are denatured by the SDS proteins have the same charge-to-mass ratio smaller proteins migrate more rapidly through the gel all of the above Answer: all of the above
