Q. In SDS-PAGE, the protein sample is first treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent treated with a oxidizing agent and then with anionic detergent followed by fractionation by electrophoresis none of the above […]
Q. In isoelectric focusing, proteins are separated on the basis of their relative content of positively charged residue only relative content of negatively charged residue only size relative content of positively and negatively charged residue Answer: relative content of positively and negatively charged residue
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Q. For the better resolution of minute protein bands in SDS-PAGE, which of the following staining method is advised? CBB Staining Silver staining Avidin staining All of these Answer: Silver staining
Q. Which of the following is used as a ‘tracking dye’ in SDS-PAGE of protein? Bromophenol blue Xylene cyanol Orange G All of these Answer: All of these
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Q. The role of APS in SDS PAGE is to________. Act as a catalyst in the polymerization of acrylamide Act as a source of free radicals Act as a bridge between acrylamide and bis-acrylamide Act as a pore builder in the polymerized gel Answer: Act as a source of free radicals
Q. The pH of (i) stacking, (ii) resolving gel and (iii) tank buffer in SDS PAGE is_________ respectively. (i). 8.30 (ii) 8.80 (iii) 6.80 (i). 6.80 (ii) 8.80 (iii) 8.30 (i). 8.30 (ii) 6.80 (iii) 8.80 Any of the above Answer: (i). 6.80 (ii) 8.80 (iii) 8.30
Q. The main advantage of discontinuous buffer system in SDS and Native PAGE is________. Conformation of protein is conserved Constantly maintain the charge of proteins Assist in migration of protein Enhance resolution of separation Answer: Enhance resolution of separation
The main advantage of discontinuous buffer system in SDS and Native PAGE is Read More »
Q. In SDS-PAGE, migration of protein is effected by ____________. Charge of protein Size of protein Net charge of protein All of these Answer: Size of protein
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Q. Sodium dodecyl sulfate (SDS) used in SDS PAGE is___________. An anionic detergent A cationic detergent A non-ionic detergent An anion exchanger Answer: An anionic detergent
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Q. For the separation of DNA by electrophoresis, which of the following method is commonly used? Agarose – vertical Agarose – horizontal PAGE – vertical PAGE – horizontal Answer: Agarose – horizontal
