Q. For the better resolution of minute protein bands in SDS-PAGE, which of the following staining method is advised? CBB Staining Silver staining Avidin staining All of these Answer: Silver staining
Q. Which of the following is used as a ‘tracking dye’ in SDS-PAGE of protein? Bromophenol blue Xylene cyanol Orange G All of these Answer: All of these
Which of the following is used as a ‘tracking dye’ in SDS-PAGE of protein? Read More »
Q. The role of APS in SDS PAGE is to________. Act as a catalyst in the polymerization of acrylamide Act as a source of free radicals Act as a bridge between acrylamide and bis-acrylamide Act as a pore builder in the polymerized gel Answer: Act as a source of free radicals
Q. The pH of (i) stacking, (ii) resolving gel and (iii) tank buffer in SDS PAGE is_________ respectively. (i). 8.30 (ii) 8.80 (iii) 6.80 (i). 6.80 (ii) 8.80 (iii) 8.30 (i). 8.30 (ii) 6.80 (iii) 8.80 Any of the above Answer: (i). 6.80 (ii) 8.80 (iii) 8.30
Q. The main advantage of discontinuous buffer system in SDS and Native PAGE is________. Conformation of protein is conserved Constantly maintain the charge of proteins Assist in migration of protein Enhance resolution of separation Answer: Enhance resolution of separation
The main advantage of discontinuous buffer system in SDS and Native PAGE is Read More »
Q. In SDS-PAGE, migration of protein is effected by ____________. Charge of protein Size of protein Net charge of protein All of these Answer: Size of protein
In SDS-PAGE, migration of protein is effected by Read More »
Q. Sodium dodecyl sulfate (SDS) used in SDS PAGE is___________. An anionic detergent A cationic detergent A non-ionic detergent An anion exchanger Answer: An anionic detergent
Sodium dodecyl sulfate (SDS) used in SDS PAGE is Read More »
Q. For the separation of DNA by electrophoresis, which of the following method is commonly used? Agarose – vertical Agarose – horizontal PAGE – vertical PAGE – horizontal Answer: Agarose – horizontal
Q. When voltage ‘V’ is applied across a pair of electrode (cathode and anode), a potential gradient ‘E’ is created between the electrodes. We can calculate ‘E’ as: E = V/d E = (1/V) x q E = (Vd)/q None of these Answer: E = V/d
Q. Pulse field gel electrophoresis was developed by ____________ Collins and John Kary Mullis Patrick O’ Farrell Schwartz and Cantor Answer: Schwartz and Cantor
Pulse field gel electrophoresis was developed by Read More »
