Q. Pulse field gel electrophoresis separates DNA molecules of size ___________ 10 – 20 bp 20 – 30 Kb 30 – 50 Kb 40 – 50 bp Answer: 30 – 50 Kb
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Q. The fluorescent dye such Ethidium is used for visualizing DNA. How do ethidium binds to DNA? Stacked between histone molecules Binds to the nucleotide base Intercalated between the stacked bases Binds to the phosphodiester backbone Answer: Intercalated between the stacked bases
Q. The polymerization of the gel used in PAGE occurs between polyacrylamide and____________ N, N – acrylamide Bisacrylamide N – methyleneacrylamide N, N – methylene bisacrylamide Answer: N, N – methylene bisacrylamide
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Q. Which of the following cannot be used for the separation of nucleic acids? SDS – PAGE PAGE Northern blotting PAGE Answer: SDS – PAGE
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Q. When is electrophoresis not used? Separation of proteins Separation of amino acids Separation of Lipids Separation of nucleic acids Answer: Separation of Lipids
Q. Which of the following factors does not influence electrophoretic mobility? Molecular weight Shape of molecule Size of molecule Stereochemistry of molecule Answer: Stereochemistry of molecule
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Q. The electrophoretic mobility denoted as µ is mathematically expressed as: VE V/E E/V 1/EV Answer: V/E
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Q. Agarose can be extracted from which of the following? Lycazusican esculentum Ficum benghalensis Gracilaria esculentus Agrostis stolonifera Answer: Gracilaria esculentus
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Q. Which of the following statements is true about migration of biomolecules? The rate of migration is directly proportional to the resistance of medium Rate of migration is directly proportional to current Low voltage is used for separation of high mass molecules Rate of migration is inversely proportional to current Answer: Rate of migration is
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Q. The speed of migration of ions in electric field depends upon: Shape and size of molecule Magnitude of charge and shape of molecule Magnitude of charge shape and mass of molecule Magnitude of charge and mass of molecule Answer: Magnitude of charge and shape of molecule
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