Applications of Biotechnology MCQs | Applications of Biotechnology Short Questions and Answers

Questions
1 Klenow fragment is derived from
A DNA Ligase
B DNA Pol-I
C DNA Pol-II
D Reverse Transcriptase

Answer:DNA Pol-I
2 Southern blotting is
A Attachment of probes to DNA fragments
B Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet
C Comparison of DNA fragments to two sources
D Transfer of DNA fragments to electrophoretic gel from cellulose membrane

Answer: Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet
3 ELISA is
A Using radiolabelled second antibody
B Usage of RBCs
C Using complement-mediated cell lysis
D Addition of substrate that is converted into a coloured end product

Answer: Addition of substrate that is converted into a coloured end product
4 The Golden Rice variety is rich in
A Vitamin C
B Β-carotene and ferritin
C Biotin
D Lysine

Answer:Β-carotene and ferritin
5 The DNA fragments have sticky ends due to
A Endonuclease
B Unpaired bases
C Calcium ions
D Free methylation

Answer:Unpaired bases
6 Plasmids are used as cloning vectors for which of the following reasons?
A Can be multiplied in culture
B Self-replication in bacterial cells
C Can be multiplied in laboratories with the help of enzymes
D Replicate freely outside bacterial cells

Answer: Self-replication in bacterial cells
7 The human genome project was launched in the year
A 1980
B 1973
C 1990
D 1989

Answer: 1990
8 The vaccines prepared through recombinant DNA technology are
A Third generation vaccines
B First-generation vaccines
C Second-generation vaccines
D None

Answer:Third generation vaccines
9 Which is a genetically modified crop?
A Bt-cotton
B Bt-brinjal
C Golden rice
D All

Answer: All
10 PCR technique was invented by
A Karry Mullis
B Boyer
C Sanger
D Cohn

Answer: Karry Mullis
11 The first transgenic plant to be produced is
A Brinjal
B Tobacco
C Rice
D Cotton

Answer: Tobacco
12 RNA interference helps in
A Cell proliferation
B Micropropagation
C Cell defence
D Cell differentiation

Answer: Cell defence
13 Which of the following is the quality of improved transgenic basmati rice?
A Gives high yield but no characteristic aroma
B Gives high yield and is rich in vitamin A
C Does not require chemical fertilizers and growth hormones
D Resistant to insects and diseases

Answer: Gives high yield and is rich in vitamin A
14 The first clinical application of gene therapy over a 4 year old girl was for
A Adenosine deaminase deficiency
B Adenosine deficiency
C Growth deficiency
D Adenine deficiency

Answer: Adenosine deaminase deficiency
15 Excision and insertion of a gene is called
A Biotechnology
B Genetic engineering
C Cytogenetics
D Gene therapy

Answer:Genetic engineering
16 The expression of a transgene in the target tissue is identified by a
A Transgene
B Promoter
C Enhancer
D Reporter

Answer:Reporter
17 ———– is used as a vector for cloning into higher organisms
A Retrovirus
B Baculovirus
C Salmonella typhimurium
D Rhizopus nigricans

Answer: Retrovirus
18 Which bacterium is used in the production of insulin by genetic engineering?
A Saccharomyces
B Rhizobium
C Escherichia
D Mycobacterium

Answer:Escherichia
19 Golden rice is a transgenic crop of the future with the following improved trait.
A Insect resistance
B High protein content
C High vitamin A content
D High lysine content

Answer: High vitamin A content
20 DNA fingerprinting refer to
A Techniques used for identification of fingerprints of individuals
B Molecular analysis of profiles of DNA samples
C Analysis of DNA samples using imprinting devices
D Techniques used for molecular analysis of different specimens of DNA

Answer: Molecular analysis of profiles of DNA samples
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