Electrophoresis Quiz | Electrophoresis Objective Type Questions and Answers

Questions
21 Which of the following is used as a ‘tracking dye’ in SDS-PAGE of protein?
A Bromophenol blue
B Xylene cyanol
C Orange G
D All of these

Answer: All of these
22 For the better resolution of minute protein bands in SDS-PAGE, which of the following staining method is advised?
A CBB Staining
B Silver staining
C Avidin staining
D All of these

Answer: Silver staining

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23 In a gel filtration column
A smaller proteins enter the beads more readily
B large proteins elute first
C both (1) and (2)
D large proteins enter the beads more readily

Answer: both (1) and (2)
24 In isoelectric focusing, proteins are separated on the basis of their
A relative content of positively charged residue only
B relative content of negatively charged residue only
C size
D relative content of positively and negatively charged residue

Answer: relative content of positively and negatively charged residue
25 In SDS-PAGE, the protein sample is first
A treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis
B fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent
C treated with a oxidizing agent and then with anionic detergent followed by fractionation by electrophoresis
D none of the above

Answer: treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis
26 In an SDS-PAGE
A proteins are denatured by the SDS
B proteins have the same charge-to-mass ratio
C smaller proteins migrate more rapidly through the gel
D all of the above

Answer: all of the above
27 In a native PAGE, proteins are separated on the basis of
A net negative charge
B net charge and size
C net positive charges size
D net positive charge

Answer: net charge and size
28 The role of urea in PAGE separation of DNA is to _______.
A Act as anion
B Act as cation
C Helps to denature the DNA
D Provide buffer stability of the gel

Answer: Helps to denature the DNA
29 In native-PAGE the separation of protein is influenced by ___________.
A Charge of protein
B Size of protein
C pI of protein
D Both (a) and (b)

Answer: Both (a) and (b)
30 In electrophoresis, the electrophoretic mobility (‘µ’) is determines the characteristics of migration of different biomolecules. Which of the following is not having any influence in ‘µ’?
A Size of molecule
B Shape of molecule
C Molecular weight
D Stereochemistry of molecule

Answer: Stereochemistry of molecule
31 Function of β-mercaptoethanol in SDS-PAGE is__________.
A To give negative charges to amino acids in the proteins
B For the oxidation of disulfide bonds in the proteins
C For breaking hydrogen bonds in the proteins
D For the reduction of disulfide bonds in the proteins

Answer: For the reduction of disulfide bonds in the proteins
32 The ratio of velocity (‘v’) of biomolecule in a medium under constant electric field (‘E’) is called ‘Electrophoretic mobility’ denoted as ‘µ’. ‘µ’ is mathematically expressed as:
A µ = E/v
B µ = 1/(Ev)
C µ = VE
D µ = v/E

Answer: µ = v/E
33 Electrophoresis is not used for the separation of ________.
A Nucleic acids
B Proteins
C Amino acids
D Lipids

Answer: Lipids
34 In SDS-PAGE of protein separation, one SDS molecule will binds to __________.
A Every amino acid
B Every two amino acids
C Every three amino acids
D Every Four amino acids

Answer: Every two amino acids
35 Glycerol is added to protein samples before they are loaded to the ‘wells’ of PAGE. The function of glycerol is to _______.
A Stabilize protein structure
B Provide density to the sample
C Helps to bind SDS to the protein
D Helps to reduce disulfide bonds by β-mercaptoethanol

Answer: Provide density to the sample
36 What does the electrophoresis apparatus consist of?
A Gel, buffer chamber and fire pack
B Buffer chamber and electrophoresis unit
C Power pack and electrophoresis unit
D Electrophoresis unit and gel separator

Answer: Power pack and electrophoresis unit
37 What cannot be a reason for using electrophoresis?
A Comparing two sets of DNA
B Organizing DNA by shape of backbone
C Organizing DNA fragments from largest to smallest
D Organizing DNA in order we can see

Answer: Organizing DNA by shape of backbone
38 If DNA is digested by endonucleases in four sites giving rise to fragments of which two are equal in length how many bands would be seen after electrophoresis?
A 3
B 4
C 5
D Any of the above

Answer: 4
39 Which of the following will migrate faster? The condition is the molecular weight of the following is equal.
A Nicked circular DNA
B Double stranded DNA
C Single stranded DNA
D Supercoiled circular DNA

Answer: Supercoiled circular DNA
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