Molecular Biology Techniques Quiz | Molecular Biology Techniques Objective Type Questions and Answers

Questions
21 In E. Coli, Inactivation Of Cellular DNA Methylase Enzyme Causes Severe Mutation In The Genomic DNA. Which Of The Following DNA Repair Mechanism Would Be Most Probably Inhibited?
A Mismatch Repair
B Nucleotide Excision Repair
C Double Strand Break Repair
D Base Excision Repair

Answer: Mismatch Repair
22 In Northern Hybridization Probe Hybridization Forms
A RNA:DNA Hybrid
B DNA:DNA Hybrid
C Both A And B
D None Of These

Answer: Both A And B
23 All Are Differences In Procedure Between Northern And Southern Hybridization Except
A Intially Fragments Are Separated By Electrophoresis In Northern Hybridization
B DNA Denaturation Is Required Before Blotting In Sothern Hybridization
C DBM Membrane Is Used In Northern Hybridization
D RNA:DNA Hybrids Are Formed In Northern Hybridization

Answer: Intially Fragments Are Separated By Electrophoresis In Northern Hybridization
24 In Western Blotting
A Polyacrylamide Gel Is Commonly Used
B Agarose Gel Is Commonly Used
C Both A And B
D High Resolution Gels

Answer: Polyacrylamide Gel Is Commonly Used
25 For Protein Detection, Most Commonly Used Probe Is
A Interferons
B Antigens
C Lectin
D Antibody

Answer: Antibody
26 DNA Helicase Enzyme Involved In Base Excision Repair Mechanism Is______.
A DNA Helicase III
B DNA Helicase IV
C DNA Helicase I
D DNA Helicase II

Answer: DNA Helicase III
27 Xeroderma Pigmentosum In Human Is Associated With A Mutation In
A Photoreactivation
B Mismatch Repair
C Nucleotide Excision Repair
D Base Excision Repair

Answer: Nucleotide Excision Repair
28 Which Of The Following Statement Is Correct?
A Photoreactivation Is Present In All Organism
B Photoreactivation Is Present Only In Bacteria
C Photoreactivation Is Absent In Human
D None of these

Answer: Photoreactivation Is Absent In Human
29 The Activity Of AP Endonuclease Is Involved In ________.
A Double Strand Break Repair
B Base Excision Repair
C Nucleotide Excision Repair
D Mismatch Repair

Answer: Base Excision Repair
30 In Eukaryotes, The Mismatch Repair Mechanism Is Initiated And Directed By_____.
A Strand Specific Nicks
B Double Strand Breaks
C Methylated DNA Strand
D Acetylated DNA Strand

Answer: Strand Specific Nicks
31 The Two Chromophores Present In Photolyase Enzyme Are:
A FAD – And Folate
B FADH And Folate –
C NAD And FAD
D FAD And Folate

Answer: FAD – And Folate
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