Questions
1
The fluorescent dye such Ethidium is used for visualizing DNA. How do ethidium binds to DNA?
Answer:Intercalated between the stacked bases
2
Pulse field gel electrophoresis separates DNA molecules of size ___________
Answer: 30 – 50 Kb
3
Which of the following will migrate faster? The condition is the molecular weight of the following is equal.
Answer: Supercoiled circular DNA
4
Agarose can be extracted from which of the following?
Answer:Gracilaria esculentus
5
Electrophoresis cannot be used to separate _______________
Answer: Amino acid
6
Which of the following is not a character of polyacrylamide gel?
Answer:Separate upto a few 100 bp of DNA
7
Pulse field gel electrophoresis was developed by ____________
Answer: Schwartz and Cantor
8
What is the condition required to observe B form of DNA?
Answer:High humidity
11
Which of the following is not a character of A DNA?
Answer:Left handed helix
13
Which of the following is not a character of B form of DNA?
Answer: Flat major groove
14
In a right handed DNA the glycosidic bond is in syn configuration.
Answer: False
15
In the Z form of DNA the fundamental repeating unit is __________
Answer:Purine – pyrimidine tetra-nucleotide
16
What is the factor responsible for the left-handed helical conformation in Z form of DNA?
Answer:Anti conformation at purine
17
In higher salt concentration the DNA helix assumes the right-handed conformation.
Answer:False
18
The two strands of cccDNA can be easily separated without any breakage of bonds.
Answer:False
19
Passing of one strand through the other in order to get separated is known as _______
Answer: Linking number
