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Principles and Processes of Biotechnology MCQs | Principles and Processes of Biotechnology Short Questions and Answers

Questions
1 Restriction endonucleases are enzymes which
A Make cuts at specific positions within the DNA molecule
B Recognize a specific nucleotide sequence for binding of DNA ligase
C Restrict the actions of the enzyme DNA polymerase
D Remove nucleotides from the ends of the DNA molecules

Answer:Make cuts at specific positions within the DNA molecule
2 Stirred-tank bioreactors have been designed for
A Addition of preservatives to the products
B Purification of the product
C Ensuring anaerobics conditions in the culture vessel
D Availability of oxygen throughout the process

Answer:Availability of oxygen throughout the process
3 Which of the following are used in gene cloning ?
A Nucleoids
B Lomasomes
C Mesosomes
D Plasmids

Answer:Plasmids
4 In genetic engineering, a DNA segment (Gene) of interest, is transferred to the host cell through a vector. Consider the following four agents (i-iv) in this regard and select the correct option about which one or more of these can be used as a vector/vectors
i) Bacterium
ii) Plasmid
iii) Plasmodium
iv) Bacteriophage
A (i),(ii) & (iv)
B (i) only
C (i) & (iii)
D (ii) & (iv)

Answer:(ii) & (iv)
5 Given below is a simple of a portion of DNA strand giving the base sequence on the opposite strands. What is so specialshown in it ?
5’ GAATTC 3’
3’ CTTAAG 5’
A Replication completed
B Deletion mutation
C Start condon at the 5’ end
D Plindromic sequence of base pairs

Answer: Plindromic sequence of base pairs
6 There is a restriction endomolecules called Eco RI. What does “co” part in it stand for ?
A Colon
B Coelom
C Coenzyme
D Coli

Answer:Coli
7 Agarose extracted from sea weeds is used in
A Spectrophotometry
B Tissue culture
C PCR
D Gel electrophoresis

Answer:Gel electrophoresis
8 Which one of the following techniques made it possible to genetically engineer living organisms ?
A Recombinant DNA techniques
B X-ray diffraction
C Heavier isotope labeling
D Hybridizatiion

Answer:Recombinant DNA techniques
9 The given figure is the diagrammatic representation of the E.Coli vector pBR322. Which one of the given options correctly identifies its certain components(s) ? image01
A Ori-original restriction enzyme
B Rop-reduced osmotic pressure
C Hin d III, Eco RI selectable markers
D AmpR , tetR – antibiotic resistance genes

Answer: AmpR , tetR – antibiotic resistance genes
10 PCR and restriction fragment length polymorphism are the methods for
A Study of enzymes
B Genetic transformations
C DNA sequencing
D Genetic fingerprints

Answer: Genetic fingerprints

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