Principles and Processes of Biotechnology MCQs | Principles and Processes of Biotechnology Multiple Choice Questions with Answers
Questions
1
One of the key factors which makes the plasmid the vector in genetic engineering is that
Answer:Its ability to carry a foreign gene
2
Which of the following is used as a best genetic vector in plants
Answer: Agrobacterium thumifaciens
3
The polymerase chain reaction is a technique that
Answer:It is used for in vitro replication of specific DNA sequence using thermostable DNA polymerase.
4
The construction of the first recombinant DNA was done by using the native plasmid of
Answer: Salmonella typhimurium
5
Gel electrophoresis is used for
Answer: Separation of DNA fragments according to their size
6
Significant of ‘heat shoch’ method in bacterial transformation is to facilitate ?
Answer: Update of DNA through transient pores in the bacterial cell wall
7
Which of the following palindromic bare sequences in DNA can be easily cut at about the middle by some particular restriction enzyme.
Answer: 5’GAATTC 3’ : CTTAAG 5’
9
Which one of the following technique made it possible to genetically engineered living organisms ?
Answer: Recombinant DNA technique
10
There is a restriction endonucleare called EcoRI. What does ‘co’ part in it stand for ?
Answer: Coli
11
A single stand of nucleic acid tagged tagged with a radioactive molecule is called
Answer: Probe
12
For transformation, micro particles coated with DNA to be bombared with gene gun are made up of
Answer: Gold or Tungsten
14
In genetic engineering, the antibiotics are used
Answer: As selectable markers
16
Bacteria protect themselves from viruses by fragmenting viral DNA with
Answer:Endonuclease
18
Southern blotting is
Answer: Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet
19
ELISA is
Answer: Addition of substrate that is converted into a coloured end product
