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Free download in PDF Very Short Questions with Answers on Molecular Basis of Inheritance for competitive exams. These short objective type questions with answers are very important for Board exams as well as competitive exams like NEET, AIIMS, JIPMER etc. These short solved questions or quizzes are provided by Gkseries./p>
(1)
The process of electrophoresis is the key to sequencing.
(2)
The dideoxy method is also known as _____________
[A]
Maxem and Gilbert method
[B]
Autosequencing
[C]
Sanger’s enzymatic sequencing
[D]
Pyrosequencing
Answer: Sanger’s enzymatic sequencing
(3)
Which enzyme is used for the replication in case of Sanger’s method of sequencing?
[A]
Polymerase I
[B]
Smaller subunit polymerase I
[C]
Polymerase III
[D]
Larger subunit polymerase I
Answer: Larger subunit polymerase I
(4)
With respect to Sanger’s enzymatic method of sequencing pick the odd one out.
[A]
Radioactive dideoxyribonucleotides
[B]
Primers
[C]
Klenow fragment
[D]
Restriction digestion
Answer: Restriction digestion
(5)
The four reaction mixtures that were prepared, contained ____________
[A]
dCTP, dTTP, dGTP, dATP
[B]
dCTP, ddTTP, dGTP, ddATP
[C]
ddCTP, dTTP, ddGTP, dATP
[D]
dCTP, ddTTP, dGTP, dATP
Answer: dCTP, ddTTP, dGTP, dATP
(6)
ddNTP acts as the chain radioactive markers.
(7)
Strand separation for DNA sequencing is done to ____________
[A]
sort the fragments according to their size
[B]
sort the fragments according to their molecular weight
[C]
obtain consecutive sequence as per termination
[D]
obtain specific lanes of sequencing
Answer: obtain consecutive sequence as per termination
(8)
The position of band during electrophoresis indicates the dNTP used as a chain terminator in that mixture.
(9)
Automated sequencing is defined as _________
[A]
Chain termination sequencing
[B]
Radio labeled sequencing
[C]
Real time fluorescence sequencing
[D]
Pyrosequencing
Answer: Real time fluorescence sequencing
(10)
Automated sequencing uses _____________
[A]
Enzymatic process
[B]
Radiolabelling
[C]
Chain termination
[D]
Fluorescent tagging
Answer: Fluorescent tagging
(11)
No chain termination takes place in automated sequencing.
(12)
As different fluorescent tags affect the mobility of fragments thus only one type of tag is used.
(13)
With respect to automated sequencing which of the following is true?
[A]
Detected by radioactive tags
[B]
Multiple plane sequencing
[C]
Horizontal scanning one sequence per lane
[D]
Vertical reading software incorporation
Answer: Horizontal scanning one sequence per lane
(14)
Which of the following feature is required for the high sensitivity of the tags used for autosequencing?
[A]
Strong absorption at UV spectrum
[B]
Maximum emittion at different wavelengths
[C]
Different mobility shifts
[D]
Induce circularization of DNA fragments
Answer: Maximum emittion at different wavelengths
(15)
Automated sequencing is a real time one trial method of sequencing and is highly handy.
(16)
A third generation sequencing can sequence upto ____________nucleotides per day.
[A]
750000
[B]
75000
[C]
570000
[D]
57000
(17)
Semiconservative nature of replication of eukaryotic chromosome was first demonstrated by _______________
[A]
Walter Flemming on root tip cells of Vicia faba
[B]
J. Herbert Taylor, Philip Wood and Walter Hughes on root tip cells of Vicia faba
[C]
Walter Flemming on root tip cells of Phaseolus vulgaris
[D]
J. Herbert Taylor, Philip Wood and Walter Hughes on root tip cells of Phaseolus vulgaris
Answer: J. Herbert Taylor, Philip Wood and Walter Hughes on root tip cells of Vicia faba
(18)
Pick the correct pair with respect to primers used in DNA replication.
[A]
RNA primer- for prokaryotes only
[B]
DNA primer-for eukaryotes only
[C]
DNA primer- for both prokaryotes and eukaryotes
[D]
RNA primer- for both prokaryotes and eukaryotes
Answer: RNA primer- for both prokaryotes and eukaryotes
(19)
Which of the following is correctly matched with its subsequent role?
[A]
Topoisomerase II- can remove both positive and negative supercoil in the DNA duplex
[B]
Polymerase I- larger fragment responsible for exonuclease activity
[C]
DnaA protein- responsible for “melting” of the DNA double helix during replication
[D]
DnaB protein- attaches to the newly unwounded single strand of DNA to prevent folding of the strand
Answer: DnaA protein- responsible for “melting” of the DNA double helix during replication
(20)
Replication fork is the junction between the two ___________
[A]
Unreplicated DNA
[B]
Newly synthesized DNA
[C]
Newly separated DNA strands and newly synthesized DNA strands
[D]
Newly separated DNA strands and the unreplicated DNA
Answer: Newly separated DNA strands and the unreplicated DNA
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