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Free Download as PDF of the detailed solutions for class 12 Biology from NCERT or CBSE text book as per exam pattern, to help you in day to day learning. We provide all important questions and answers from chapter Biotechnology Principles and Processes of Biology.
(1)
Rising of dough is due to:
[1]
Multiplication of yeast
[2]
Production of CO2
[3]
Emulsification
[4]
Hydrolysis of wheat flour starch into sugars.
Answer: Production of CO2
(2)
Restriction’ in Restriction enzyme refers to:
[1]
Cleaving of phosphodiester bond in DNA by the enzyme
[2]
Cutting of DNA at specific position only
[3]
Prevention of the multiplication of bacteriophage in bacteria
[4]
All of the above
Answer: Prevention of the multiplication of bacteriophage in bacteria
(3)
While isolating DNA from bacteria, which of the following enzymes is not used?
[1]
Lysozyme
[2]
Ribonuclease
[3]
Deoxyribonuclease
[4]
Protease
Answer: Deoxyribonuclease
(4)
Significance of ‘heat shock’ method in bacterial transformation is to facilitate:
[1]
Binding of DNA to the cell wall
[2]
Uptake of DNA through membrane transport proteins
[3]
Uptake of DNA through transient pores in the bacterial cell wall
[4]
Expression of antibiotic resistance gene
Answer: Uptake of DNA through transient pores in the bacterial cell wall
(5)
Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?
[1]
Laboratory flask of largest capacity
[2]
A stirred-tank bioreactor without in-lets and out-lets
[3]
A continuous culture system
[4]
Any of the above
Answer: A continuous culture system
(6)
Which of the following statements does not hold true for restriction enzyme?
[1]
It recognises a palindromic nucleotide sequence
[2]
It is an endonuclease
[3]
It is isolated from viruses
[4]
It produces the same kind of sticky ends in different DNA molecules
Answer: It is isolated from viruses
(7)
In agarose gel electrophoresis, DNA molecules are separated on the basis of their:
[1]
Size only
[2]
Charge only
[3]
Charge to size ratio
[4]
All of the above
(8)
The transfer of genetic material from one bacterium to another through the mediation of a vector like virus is termed as:
[1]
Transduction
[2]
Conjugation
[3]
Transformation
[4]
Translation
(9)
Which of the following bacteria is not a source of restriction endonuclease?
[1]
Haemophilus influenzae
[2]
Escherichia coli
[3]
Agrobacterium tumefaciens
[4]
Bacillius amyloli
Answer: Agrobacterium tumefaciens
(10)
Which of the following steps are catalysed by Taq polymerase in a PCR reaction?
[1]
Denaturation of template DNA
[2]
Annealing of primers to template DNA
[3]
Extension of primer end on the template DNA
[4]
All of the above
Answer: Extension of primer end on the template DNA
(11)
Who among the following was awarded the Nobel Prize for the development of PCR
technique?
[1]
Herbert Boyer
[2]
Hargovind Khurana
[3]
Kary Mullis
[4]
Arthur Kornberg
(12)
A recombinant DNA molecule can be produced in the absence of the following:
[1]
Restriction endonuclease
[2]
DNA ligase
[3]
DNA fragments
[4]
E.coli
(13)
The technique of DNA fingerprinting was perfected by
[1]
Stahl
[2]
Beadle and Tatum
[3]
Franklin
[4]
Alec Jeffreys
(14)
Restriction endonuclease (RE) was discovered by
[1]
Meselson
[2]
Cohen
[3]
Arber
[4]
Watson
(15)
Examples for clones
[1]
Monozygotic identical twins
[2]
Vegetative reproducing organism
[3]
Sexually reproducing organisms
[4]
All except C
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