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NCERT - Biotechnology Principles and Processes Questions and Answers of Class 12 Biology | GkSeries

Questions
76 Dolly, The first cloned mammal, was produced by
1 Parthenogenesis
2 Artificial fertilization
3 Replacement of zygote nucleus by somatic nucleus
4 in vitro fertilization

Answer: Replacement of zygote nucleus by somatic nucleus
77 An improved variety of transgenic basmati rice
1 Gives high yield and is rich in vitamin A
2 Does not require chemical fertilizers and growth hormones
3 Give high yield but has no characteristic aroma
4 Is completely resistant to all insect pests and diseases of paddy

Answer: Gives high yield and is rich in vitamin A
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78 Restriction endonuclease
1 Restricts the synthesis of DNA inside the nucleus
2 Cuts the DNA molecule at specific sites
3 Cuts the DNA molecule randomly
4 Synthesizes DNA

Answer: Cuts the DNA molecule at specific sites
79 Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?
1 5′ CACGTA 3′; 3′ CTCAGT 5′
2 5′ CGTTCG 3′; 3′ ATGGTA 5′
3 5′ GATATG 3′; 3′ CTACTA 5′
4 5′ GAATTC 3′; 3′ CTTAAG 5

Answer: 5′ GAATTC 3′; 3′ CTTAAG 5
80 Plasmid has been used as vector because :
1 Both ends show replication
2 It can move between prokaryotic and eukaryotic cells
3 It is circular DNA which have capacity to join to eukaryotic DNA
4 It has antibiotic resistance geneRecombinant DNA technology

Answer: It is circular DNA which have capacity to join to eukaryotic DNA
81 Structure involved in genetic engineering is
1 Plasmid
2 Codon
3 Plastid
4 Scissors

Answer: Plasmid
82 Embryo culture is used for
1 Establishing suspension culture
2 Recovery of inter specific hybrids
3 Somatic hybridization
4 Haploid production

Answer: Recovery of inter specific hybrids
83 A genetically engineered micro-organism used successfully in bioremediation of oil spills is a species of
1 Trichoderma
2 Pseudomonas
3 Bacillus
4 Xanthomonas

Answer: Pseudomonas
84 Which one of the following is used as vector for cloning genes into higher organisms?
1 Salmonella typhimurium
2 Retrovirus
3 Baculovirus
4 Neurospora crassa

Answer: Retrovirus
85 The enzymes required to obtain wall-free / naked protoplasts are
1 Cellulase and proteinase
2 Cellulase and pectinase
3 Cellulase and amylase
4 Amylase and pectinase

Answer: Cellulase and pectinase
86 The first clinical gene therapy was given for treating?
1 Rheumatoid arthritis
2 Chicken pox
3 Diabetes mellitus
4 Adenosine deaminase deficiency

Answer: Adenosine deaminase deficiency
87 Adenosine deaminase deficiency
1 foreign DNA in some of their cells
2 foreign DNA in all of their cells
3 foreign RNA in all of their cells
4 Both (A) and (C)

Answer: foreign DNA in all of their cells
88 The vector for T-DNA is
1 Thermus aquaticus
2 Salmonella typhimurium
3 Agrobacterium tumefaciens
4 Bacillus thuringiensis

Answer: Agrobacterium tumefaciens
89 First artificial gene was synthesized by
1 Nirenberg
2 Mendel
3 Morgan
4 Khorana

Answer: Khorana
90 Genetic Engineering is
1 plastic surgery
2 addition or removal of genes
3 study of extranuclear genes
4 all of the above

Answer: addition or removal of genes
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