NCERT - Biotechnology Principles and Processes Questions and Answers of Class 12 Biology | GkSeries

Questions
61 Genetic engineering has been successfully used for producing
1 Transgenic models for studying new treatments for certain cardiac diseases
2 Transgenic mice for testing safety of polio vaccine before use in humans
3 Animals like bulls for farm work as they have super power
4 Transgenic Cow-Rosie which produces high fat milk for making ghee

Answer: Transgenic mice for testing safety of polio vaccine before use in humans
62 Which one contains four pyrimidine bases?
1 GATCAATGC
2 GCUAGACAA
3 UAGCGGUAA
4 TGCCTAACG

Answer: GATCAATGC

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63 Which of the following is correctly matched?
1 Ligase - Molecular scissors
2 Thermus aquaticus - Bt-gene
3 Agrobacterium tumefaciens - Tumour
4 Hind II - Plasmid vector

Answer: Agrobacterium tumefaciens - Tumour
64 The specific triplet of nitrogen bases on the transfer RNA is called
1 Codon
2 Anticodon
3 Genetic code
4 none of the above

Answer: Anticodon
65 Restriction endonucleases:
1 Are synthesized by bacteria as part of their defense mechanism
2 Are used for in vitro DNA synthesis
3 Are used in genetic engineering for ligation of two DNA molecules
4 Are present in mammalian cells for degradation of DNA when the cell dies

Answer: Are synthesized by bacteria as part of their defense mechanism
66 Restriction endonucleases are used in genetic engineering and theyRestriction endonucleases are used in genetic engineering and they
1 cut DNA at various sites
2 join DNA segments
3 cut RNA at specific sites
4 cut DNA at specific sites

Answer: cut DNA at specific sites
67 DNA fingerprinting was discovered by
1 James Watson
2 Alec Jeffreys
3 Frederick Fanger
4 Hargobind Khorana

Answer: Alec Jeffreys
68 Which of the following enzymes are used to join bits of DNA?
1 DNA polymerase
2 Primase
3 Ligase
4 Endonuclease

Answer: Ligase
69 Sterilization of tissue culture medium is done by
1 mixing the medium with antifungal agents
2 filtering the medium through fine sieve
3 autoclaving of medium at 120° for 15 min
4 keeping the medium at –20°C

Answer: autoclaving of medium at 120° for 15 min
70 Which one is found only in RNA?
1 Cytosine
2 Adenine
3 Uracil
4 Guanine

Answer: Uracil
71 The term gene was coined by
1 Mc Clintock
2 Morgan
3 Johannsen
4 De Duve

Answer: Johannsen
72 Biolistics (gene-gun) is suitable for
1 Constructing recombinant DNA by joining with vectors
2 Transformation of plant cells
3 Disarming pathogen vectors
4 DNA finger printing

Answer: Transformation of plant cells
73 Restriction endonucleases are used in genetic engineering, because
1 they can degrade harmful proteins
2 they can join DNA fragments
3 they can cut DNA at specific base sequences
4 they can cut DNA at variable sites

Answer: they can cut DNA at specific base sequences
74 Which one of the following bacteria has found extensive use in genetic engineering work in plants?
1 Bacillus coagulans
2 Xanthomonas citri
3 Clostridium septicum
4 Agrobacterium tumefaciens

Answer: Agrobacterium tumefaciens
75 DNA is found in
1 Chromosomes
2 Plastids
3 Mitochondria
4 All of the above

Answer: All of the above

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