NCERT - Biotechnology Principles and Processes Questions and Answers of Class 12 Biology | GkSeries

61 Genetic engineering has been successfully used for producing
[1] Transgenic models for studying new treatments for certain cardiac diseases
[2] Transgenic mice for testing safety of polio vaccine before use in humans
[3] Animals like bulls for farm work as they have super power
[4] Transgenic Cow-Rosie which produces high fat milk for making ghee
Answer: Transgenic mice for testing safety of polio vaccine before use in humans
62 Which one contains four pyrimidine bases?
[1] GATCAATGC
[2] GCUAGACAA
[3] UAGCGGUAA
[4] TGCCTAACG
Answer: GATCAATGC

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63 Which of the following is correctly matched?
[1] Ligase - Molecular scissors
[2] Thermus aquaticus - Bt-gene
[3] Agrobacterium tumefaciens - Tumour
[4] Hind II - Plasmid vector
Answer: Agrobacterium tumefaciens - Tumour
64 The specific triplet of nitrogen bases on the transfer RNA is called
[1] Codon
[2] Anticodon
[3] Genetic code
[4] none of the above
Answer: Anticodon
65 Restriction endonucleases:
[1] Are synthesized by bacteria as part of their defense mechanism
[2] Are used for in vitro DNA synthesis
[3] Are used in genetic engineering for ligation of two DNA molecules
[4] Are present in mammalian cells for degradation of DNA when the cell dies
Answer: Are synthesized by bacteria as part of their defense mechanism
66 Restriction endonucleases are used in genetic engineering and theyRestriction endonucleases are used in genetic engineering and they
[1] cut DNA at various sites
[2] join DNA segments
[3] cut RNA at specific sites
[4] cut DNA at specific sites
Answer: cut DNA at specific sites
67 DNA fingerprinting was discovered by
[1] James Watson
[2] Alec Jeffreys
[3] Frederick Fanger
[4] Hargobind Khorana
Answer: Alec Jeffreys
68 Which of the following enzymes are used to join bits of DNA?
[1] DNA polymerase
[2] Primase
[3] Ligase
[4] Endonuclease
Answer: Ligase
69 Sterilization of tissue culture medium is done by
[1] mixing the medium with antifungal agents
[2] filtering the medium through fine sieve
[3] autoclaving of medium at 120° for 15 min
[4] keeping the medium at –20°C
Answer: autoclaving of medium at 120° for 15 min
70 Which one is found only in RNA?
[1] Cytosine
[2] Adenine
[3] Uracil
[4] Guanine
Answer: Uracil
71 The term gene was coined by
[1] Mc Clintock
[2] Morgan
[3] Johannsen
[4] De Duve
Answer: Johannsen
72 Biolistics (gene-gun) is suitable for
[1] Constructing recombinant DNA by joining with vectors
[2] Transformation of plant cells
[3] Disarming pathogen vectors
[4] DNA finger printing
Answer: Transformation of plant cells
73 Restriction endonucleases are used in genetic engineering, because
[1] they can degrade harmful proteins
[2] they can join DNA fragments
[3] they can cut DNA at specific base sequences
[4] they can cut DNA at variable sites
Answer: they can cut DNA at specific base sequences
74 Which one of the following bacteria has found extensive use in genetic engineering work in plants?
[1] Bacillus coagulans
[2] Xanthomonas citri
[3] Clostridium septicum
[4] Agrobacterium tumefaciens
Answer: Agrobacterium tumefaciens
75 DNA is found in
[1] Chromosomes
[2] Plastids
[3] Mitochondria
[4] All of the above
Answer: All of the above

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